Docetaxel trihydrate

SKU: orb1310857

Description

Docetaxel trihydrate

Research Area

Cell Biology, Signal Transduction

Key Properties

• CAS Number: 148408-66-6
• MW: 861.95
• Purity: 99.84%
• Formula: C₄₃H₅₉NO₁₇
• SMILES: O.O.O.CC(=O)O[C@@]12CO[C@@H]1C[C@H](O)[C@]1(C)[C@@H]2[C@H](OC(=O)c2ccccc2)[C@]2(O)C[C@H](OC(=O)C(O)C(NC(=O)OC(C)(C)C)c3ccccc3)C(C)=C([C@@H](O)C1=O)C2(C)C
• Target: Microtubule Associated,Apoptosis,Bcl-2 Family
• Solubility: Ethanol:100 mg/mL (116.02 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:4 mg/mL (4.64 mM);H2O:< 1 mg/mL (insoluble or slightly soluble);DMSO:100 mg/mL (116.02 mM)

Bioactivity

• In Vivo:
  Docetaxel inhibit the clonogenic survival of various human cancer cell lines including Hs746T (stomach), AGS (stomach), HeLa (cervical), CaSki (cervical), BxPC3 (pancreatic), and Capan-1 (pancreatic) with IC50 Values of 1 nM fo the stomach, and 0.3 nM fo the others, respectively Additionally, Docetaxel suppresse the chemotaxis of human umbilical vein endothelial cells stimulated by angiogenic factors, thymidine phosphorylase, or VEGF, with an IC50 of 10 pM. Moreover, Docetaxel induce the expression of Prostag andin H Synthase-2 (PGHS-2) in human monocytes without affecting its expression in RAW 264.7 mouse macrophages.
• In Vitro:
  Administering 33 mg/kg of Docetaxel every four days for three doses resulted in a 19.3-day growth delay in M2OL2 colorectal cancer xenografts. Docetaxel exhibited potent antitumor activity in MX-1, SK-MEL-2, LX-1, and OVCAR-3 xenograft models. Biweekly administration of Docetaxel over 14 days suppressed fibroblast growth factor 2-mediated angiogenesis with an IC50 of 5.4 mg/kg. A dose of 10 mg/kg Docetaxel completely inhibited angiogenesis in mice.
• Cell Research:
  2000 cells in 180 μL of medium are seeded in a 96-well plate, and 20 μL of drug solution is simultaneously added in triplicate to each well the plate is incubated for 3 days at 37℃n a humidified atmosphere of 5% CO2. On day 3, 25 μL of MTT reagent is added to Each well. After 4 h of incubation the medium is removed by aspiration. 0.2 ml of dimethylsulphoxide (DMSO) is added to each well and thoroughly mixed by using a Mechanical plate mixer to dissolve the resulting MTT-formazan crystals. Absorbance at 540 nM (OD) is measured by a reader. (Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

Docetaxel, Docetaxel trihydrate, Docetaxel Trihydrate, Apoptosis, Bcl-2, inhibit, Microtubule/Tubulin, MicrotubuleAssociated, Microtubule Associated, Inhibitor, Tubulin β-1 chain, RP 56976, RP56976, RP-56976, RP56976 (NSC 628503), RP56976 (NSC 628503) Trihydrate, RP-56976 (Trihydrate)

Compound Identifiers

PubChem CID

86280293