DNMT1 Antibody

SKU: orb67388

Description

Mouse Anti-Human DNMT1 Monoclonal IgG1 Kappa

Research Area

Cell Biology, Protein Biochemistry, Signal Transduction

Images & Validation

• Tested Applications: ChIP, IHC, IP, WB
• Dilution Range: WB (1:1000), IHC-P (1:1000)
• Reactivity: Fish, Human, Mouse, Zebrafish
• Application Notes:
  2 µg/ml of SMC-201 was sufficient for detection of Dnmt1 in 10 µg of mouse ES cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1 Kappa
• Clone No.: 4G11-C7
• Immunogen: Raised against a partial protein corresponding to amino acids 620-950 of human DNMT1
• Target: DNMT1
• Molecular Weight: 180kDa
• Purification: Protein G Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNA Mtase, DNMT, MCMT, DNA methyltransferase 1, AIM, CXXC9, DNA (cytosine-5)-methyltransferase 1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI

Western Blot analysis of Human H1299 cell lysate showing detection of DNMT1 protein using Mouse Anti-DNMT1 Monoclonal Antibody, Clone 4G11-C7. Primary Antibody: Mouse Anti-DNMT1 Monoclonal Antibody at 1:1000.

Quick Database Links

Gene Symbol

DNMT1

UniProt

P26358

NCBI Gene ID

1786

RefSeq (Protein):NP_001370