Dna Ligase T4

SKU: orb348601

Description

Dna Ligase T4

Research Area

Molecular Biology

Images & Validation

• Application Notes:
  One unit will catalyze the exchange of one nanomole 32-P from pyrophosphate into a Norit-absorbable compound in 20 minutes at 37° C (Weiss, B., et al., JBC 243, 4543 (1968). 50 mM Tris-HCl, 10 mM MgCl2, 10 mM DTT, 1 mM ATP, pH 7.5 @ 25°C.

Key Properties

• Biological Activity: 6.0 units/µL
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
• Form/Appearance: Liquid
• Buffer/Preservatives: Buffer: See application note.
• Concentration: 6 weiss units/µL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Hazard Information: Non-Toxic
• Disclaimer: For research use only

Alternative Names

DNA Ligase T4 Molecular Biology Grade