DNA/ EcoRI Digest

• Catalog Number: orb65519
• Category: Tools
• Description: The EcoRI digest of λ DNA yields the following 6 discrete fragments (in base pairs): 21226*, 7421, 5804, 5643, 4878, 3530*. The cohesive ends (12b cos site of bacteriophage lambda) of fragments 21226 bp and 3530 bp may anneal and form an additional band. These fragments may be separated again by heating to 65 °C for 5 min and then cooling on ice for 3 min.
• Concentration: 50 ng/µl
• Form/Appearance: liquid (supplied in Jena Bioscience 6x Green Gel Loading Buffer (#PCR-254-gr))
• Storage: store at -20 °C
• Note: For research use only
• Application notes: Preparation: Lambda DNA (48.502 bp) was completely digested by EcoRI, phenol extracted, ethanol precipitated, dissolved in 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA. ; Recommended Load: 2-5 µl per lane.
• Expiration Date: 12 months from date of receipt.