Dna Calf Thymus

SKU: orb348599

Description

Dna Calf Thymus

Images & Validation

• Application Notes:
  This product is a convenient, ready-to-use solution of calf thymus DNA especially prepared for use in the preparation of pre-hybridization and hybridization solutions and as a DNA carrier in yeast transformation protocols and other related methods. This product is also an excellent substrate for deoxyribonuclease or DNA polymerase. This solution contains sheared single stranded DNA molecules that can be used to block the non-specific attachment of probe DNA to the surface of a membrane (Southern) or to increase yeast transformation efficiency. Prepared by a modification of the method of Emanuel and Chaikoff, JBC 203, 164 (1953) from calf thymus DNA by mechanical shearing and heat denaturation to an average size of 100 to 2000 base pairs. To reverse any renaturation occurring during storage this material should be briefly boiled and rapidly chilled prior to use.

Key Properties

• Purity: Specially prepared from Calf thymus by a method to remove contaminating RNA and protein.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vials at -20° C upon receipt. Expiration date is six (6) months from date of opening vial.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Buffer: 0.01 M Tris Chloride, 0.001 M EDTA, pH 7.6
• Concentration: 5.0 mg/mL
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Hazard Information: Non-Toxic
• Disclaimer: For research use only

Alternative Names

DNA Calf Thymus, ready-to-use solution of calf thymus DNA

Absorption spectra of 50 µm [Cu(HBnz2)Cl] in 5 mM Tris-HCl buffer at pH 7.1, in the absence (R = 0) and presence of increasing amounts of CT-DNA (p/n orb348599). The ratio, R ([DNA]/[Cu(HBnz2)Cl]) = 10, 15, 20, 25, 30. Inset: expanded view of absorption changes of 50 µm [Cu(HBnz2)Cl] in the presence of increasing amounts of DNA at the respective R values.

Viscosity changes of calf thymus DNA (p/n orb348599) 1mM with increasing concentrations (0.1mM to 1mM) of ethidium bromide (square), NiCR-2H (circle), and NiCR (triangle), respectively. Experimental conditions: 10 mM Phosphate buffer pH7.0 at 25°C.