DL-Sulforaphane N-acetyl-L-cysteine

SKU: orb1693588

Description

DL-Sulforaphane N-acetyl-L-cysteine (SFN-NAC) is a sulforaphane metabolite that induces apoptosis in NSCLC cells. Its mechanism involves downregulating α-tubulin and activating ERK1/2 to phosphorylate Stathmin-1, while simultaneously upregulating Hsp70 via ERK1/2 phosphorylation. This compound is a useful research tool for studying apoptosis and kinase signaling pathways in cancer models, both in vitro and in vivo.

Research Area

Pharmacology & Drug Discovery

Key Properties

• CAS Number: 334829-66-2
• MW: 340.48
• Purity: 99.00% (May vary between batches)
• Formula: C₁₁H₂₀N₂O₄S₃
• SMILES: [C@@H](CSC(NCCCCS(C)=O)=S)(NC(C)=O)C(O)=O
• Target: HSP,ERK,Apoptosis,HDAC,Drug Metabolite
• Solubility: DMSO:8 mg/mL (23.5 mM);PBS (pH 7.2):8 mg/mL (23.5 mM);Ethanol:4 mg/mL (11.75 mM);DMF:8 mg/mL (23.5 mM)

Bioactivity

• Target IC50:
  U-87MG cells:35.20 μM|HA cells:60.08 μM|U-373MG cells:39.11 μM
• In Vivo:
  In the HDAC inhibition model, DL-Sulforaphane N-acetyl-L-cysteine (10 μmol, oral gavage, single dose, 6-hour treatment) significantly inhibited histone deacetylase (HDAC) activity in the colon mucosa of mice.
• In Vitro:
  In studies on U87MG and U373MG cells, treatment with DL-Sulforaphane N-acetyl-L-cysteine (30 μM) for 24 hours resulted in cell cycle analysis showing drug-induced G2/M phase arrest, along with the initiation of apoptosis. In the same cell lines, Western blot analysis revealed that DL-Sulforaphane N-acetyl-L-cysteine, within a concentration range of 10 to 50 μM, activated ERK1/2 (Thr202/Tyr204), downregulated α-tubulin, and induced autophagy in a dose-dependent manner. Additionally, cell viability assays showed that DL-Sulforaphane N-acetyl-L-cysteine, within a concentration range of 0 to 90 μM, decreased the viability of HA cells, U87MG cells, and U373MG cells in a dose-dependent manner, with IC50 values of 60.08 μM, 35.20 μM, and 39.11 μM, respectively.

Storage & Handling

• Storage: store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SFNNAC, SFN-NAC, DLSulforaphane NacetylLcysteine, DL-Sulforaphane N-acetyl-L-cysteine, DL Sulforaphane N acetyl L cysteine

Compound Identifiers

PubChem CID

71772353