DIM-C-pPhOH

SKU: orb1222662

Description

DIM-C-pPhOH is a Nur77 Antagonist. DIM-C-pPhOH inhibits lung cancer cell and tumor growth in a metastasis model.(In Vitro):DIM-C-pPhOH (7.5-20 μM; 24 hours; ACHN and 786-O cells) treatment significantly decreases cell proliferation.DIM-C-pPhOH (20 μM; 24 hours; ACHN and 786-O cells) treatment induces Annexin V staining in ACHN and 786-O cells, confirming that DIM-C-pPhOH induce apoptosis, and also induces cleavage of caspases 7 and 8.DIM-C-pPhOH (15-20 μM; 24 hours; ACHN and 786-O cells) treatment inhibits NR4A1-regulated expression of survivin, bcl-2 and EGFR in ACHN and 786-O cells. And also induces sestrin 2, activates AMPKα and inhibits activation of mTOR and downstream kinases.DIM-C-pPhOH decreases expression of β1-integrin protein and mRNA and β1-integrin-dependent responses in MCF7, MDA-MB-231, and SKBR3 cells and also inhibits migration of the latter two cell lines.(In Vivo):DIM-C-pPhOH (30 mg/kg; oral gavage; daily; for 50 days; male athymic nude mice) treatment results in a significant inhibition of tumor growth.

Key Properties

• CAS Number: 151358-47-3
• MW: 338.41
• Purity: >98% (HPLC)
• Formula: C₂₃H₁₈N₂O
• SMILES: Oc1ccc(cc1)C(c3cnc2ccccc23)c5cnc4ccccc45
• Target: Antioxidant
• Solubility: In Vitro: DMSO : 125 mg/mL (369.39 mM)

Bioactivity

• In Vivo:
  DIM-C-pPhOH (30 mg/kg; oral gavage; daily; for 50 days; male athymic nude mice) treatment results in a significant inhibition of tumor growth. Animal model: Male athymic nude mice (aged 6-7 weeks) injected with ACHN cells. Dosage: 30 mg/kg/day. Administration: Oral gavage; daily; for 50 days. Result: Resulted in a significant inhibition of tumor growth.
• In Vitro:
  DIM-C-pPhOH (7.5-20 μM; 24 hours; ACHN and 786-O cells) treatment significantly decreases cell proliferation. DIM-C-pPhOH (20 μM; 24 hours; ACHN and 786-O cells) treatment induces Annexin V staining in ACHN and 786-O cells, confirming that DIM-C-pPhOH induce apoptosis, and also induces cleavage of caspases 7 and 8.DIM-C-pPhOH (15-20 μM; 24 hours; ACHN and 786-O cells) treatment inhibits NR4A1-regulated expression of survivin, bcl-2 and EGFR in ACHN and 786-O cells. And also induces sestrin 2, activates AMPKα and inhibits activation of mTOR and downstream kinases. DIM-C-pPhOH decreases expression of β1-integrin protein and mRNA and β1-integrin-dependent responses in MCF7, MDA-MB-231, and SKBR3 cells and also inhibits migration of the latter two cell lines. Cell Proliferation Assay Cell line: ACHN and 786-O cells. Concentration: 7.5 μM, 15 μM, 20 μM Incubation time: 24 hours. Result: Significantly decreased cell proliferation. Apoptosis Analysis Cell line: ACHN and 786-O cells. Concentration: 20 μM. Incubation time: 24 hours. Result: Induced apoptosis in ACHN and 786-O cells. Western blot analysis. Cell line: ACHN and 786-O cells. Concentration: 15 μM, 20 μM. Incubation time: 24 hours. Result: Inhibited NR4A1-regulated expression of survivin, bcl-2 and EGFR in ACHN and 786-O cells. And also induced sestrin 2, activated AMPKα and inhibited activation of mTOR and downstream kinases.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DIM-C-pPhOH

Quick Database Links

Gene Symbol

Antioxidant