DIM-C-pPhCO2Me

SKU: orb1302005

Description

DIM-C-pPhCO2Me is a small molecule antagonist of the nuclear receptor NR4A1 (Nur77). It is used in research to investigate the receptor's role in cancer, inflammation, and metabolism in both cellular and animal models.

Research Area

Cell Biology, Metabolism Research

Key Properties

• CAS Number: 151358-48-4
• MW: 380.44
• Purity: 99.86% (May vary between batches)
• Formula: C₂₅H₂₀N₂O₂
• SMILES: COC(=O)c1ccc(cc1)C(c1c[nH]c2ccccc12)c1c[nH]c2ccccc12
• Target: PERK,Others,NR4A,Apoptosis,Isocitrate Dehydrogenase (IDH),DAPK
• Solubility: DMSO:50 mg/mL (131.43 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (5.26 mM)

Bioactivity

• In Vitro:
  DIM-C-pPhCO2Me inhibited growth and induced apoptosis in ACHN and 786-O cells . DIM-C-pPhCO2Me decreased NR4A1-dependent transactivation in RMS cells and inhibited RMS cell and tumor growth and induced apoptosis. Both NR4A1 knockdown and treatment with DIM-C-pPhCO2Me also induced ROS which activated stress genes and induced sestrin 2 which activated AMPK and inhibited mTOR in the mutant p53 RMS cells .
• Cell Research:
  Knockdown of NR4A1 in ACHN and 786-O cells was carried out using Lipofectamine 2000 reagent according to the manufacturer's protocol. siRNA complexes used in the study are as follows: siGL2-5', CGU ACG CGG AAU ACU UCG A; siNR4A1 (1)-SASI_Hs02_00333289; siNR4A1 (2)-SASI_Hs01_00182072. ACHN RCC cells were plated on 12-well plates at 5 x 104 cells per well in DMEM supplemented with 2.5% charcoal-stripped FBS. After 24 hr, various amounts of DNA [NBRE3-luc (400 ng), FLAG-NR4A1 (40 ng)] were cotransfected into each well by Lipofectamine 2000 reagent according to the manufacturer's protocol. After 6 hr of transfection, cells were treated with 2.5% stripped DMEM containing either DMSO, DIM-C-pPhOH (20 μM) or DIM-C-pPhCO2Me (15 μM) for 18 hr .

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NR4A1, Inhibitor, inhibit, DIMCpPhCO2Me, DIM-C-pPhCO2Me, DIM-C-pPhCO-2Me, DIM C pPhCO2Me

Compound Identifiers

PubChem CID

15637614