DIF-3

SKU: orb1689538

Description

DIF-3 is a research compound that activates GSK-3β, leading to the degradation of cyclin D1 and c-Myc and inhibition of Wnt/β-catenin signaling. It demonstrates antiproliferative effects in vitro, such as in HeLa and DLD-1 cancer cell lines, making it a tool for studying oncogenic pathways.

Research Area

Cell Biology, Signal Transduction, Stem Cell & Developmental Biology

Key Properties

• CAS Number: 113411-17-9
• MW: 272.72
• Purity: 99.57% (May vary between batches)
• Formula: C₁₃H₁₇ClO₄
• SMILES: CCCCCC(=O)c1c(O)cc(OC)c(Cl)c1O
• Target: Wnt/beta-catenin,CDK,GSK-3
• Solubility: 10% DMSO+90% Corn Oil:3.3 mg/mL (12.1 mM);DMSO:90 mg/mL (330.01 mM)

Bioactivity

• In Vitro:
  In this study, we examined the effects of six DIF analogues (DIF-1, DIF-2 (which has pentanone in place of hexanone), DIF-3 (dechlorinated form of DIF-1), 2-MIDIF-1 (2-methoxy isomer of DIF-1), DMPH (dechlorinated form of DIF-3), and THPH (4-hydroxy substitution of DMPH)) on DNA synthesis, cell growth, erythroid differentiation, and cytosolic free calcium concentration ([Ca2+]i) in human leukemia K562 cells. DIF-3 proved to be the most potent anti-leukemic agent among them, and the order of potency for causing growth inhibition, erythroid induction, and increases in [Ca2+]iTHPH in all the categories tested. DIF-3 activates GSK-3beta to accelerate the proteolysis of cyclin D1 and that this mechanism is involved in the DIF-3-induced G(0)/G(1) arrest in mammalian cells.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GSK-3β, GSK3, betacatenin, beta-catenin, Cyclin D1, bcatenin, βcatenin, Wnt/β-catenin, Wnt, Wnt/betacatenin, Wnt/b-catenin

Compound Identifiers

PubChem CID

3081033