Didanosine

SKU: orb1224212

Description

Didanosine is a reverse transcriptase inhibitor with an IC50 of 0.49 μM.(In Vitro):Didanosine is converted by cellular enzymes to the active antiviral metabolite dideoxyadenosine triphosphate (dd-ATP) and the intracellular half life of dd-ATP varies from 8-24 hours.Didanosine shows antiretroviral activity with IC50 value of 0.24-0.6 mg/L for HIV infection.Didanosine (5, 10, 50, 100 ug/ml; 24, 48 h) shows no significant inhibition of cell proliferation in IEC-6 cells.(In Vivo):Didanosine (100, 150 mg/kg; p.o.; daily for 7 days) decreases the length of duodenal and in jejunal villus in mouse.

Key Properties

• CAS Number: 69655-05-6
• MW: 236.23
• Purity: >98% (HPLC)
• Formula: C₁₀H₁₂N₄O₃
• SMILES: C1C[C@@H](O[C@@H]1CO)N2C=NC3=C2NC=NC3=O
• Target: Reverse Transcriptase
• Solubility: DMSO: 47 mg/mL (198.95 mM)

Bioactivity

• In Vivo:
  Didanosine (100, 150 mg/kg; p.o. ; daily for 7 days) decreases the length of duodenal and in jejunal villus in mouse. Animal model: 30-40 g, male swiss mice. Dosage: 100, 150 mg/kg. Administration: p.o. ; daily for 7 days. Result: Caused significant reductions in duodenal and in jejunal villus length and significantly decreased ileal crypt depth.
• In Vitro:
  Didanosine is converted by cellular enzymes to the active antiviral metabolite dideoxyadenosine triphosphate (dd-ATP) and the intracellular half life of dd-ATP varies from 8-24 hours. Didanosine shows antiretroviral activity with IC50 value of 0.24-0.6 mg/L for HIV infection. Didanosine (5, 10, 50, 100 ug/ml; 24, 48 h) shows no significant inhibition of cell proliferation in IEC-6 cells. Cell Proliferation Assay Cell line: IEC-6 cells. Concentration: 5, 10, 50, 100 ug/ml. Incubation time: 24, 48 h. Result: Did not show any significant inhibition of cell proliferation at either 24 h or 48 h. Apoptosis Analysis Cell line: IEC-6 cells. Concentration: 100 ug/ml. Incubation time: 24 h. Result: Induced apoptosis with the apoptosis rates of 4.7% to 7.4%.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NSC 612049

Quick Database Links

Gene Symbol

Reverse Transcriptase