Diclofenac sodium

SKU: orb1226145

Description

Diclofenac Sodium is a non-selective COX inhibitor with IC50 of 60 and 220 nM for ovine COX-1 and -2, respectively.(In Vitro):Diclofenac effectively blocks COX-1 mediated prostanoid production from U937 cell microsomes, with an IC50 of 7±3 nM.Diclofenac (1-60 μM; 1 day) induces neural stem cells (NSCs)death in a concentration-dependent manner.Diclofenac (10-60 μM; 6 hours) increases the expression of cleaved (activated) caspase-3.(In Vivo):Diclofenac (3 mg/kg, b.i.d., for 5 days) significantly increases faecal 51Cr excretion in rats, and such effect is also observed in squirrel monkeys after administrated of 1 mg/kg twice daily for 4 days.Diclofenac (10 mg/kg; administered via oral route just prior to induction of inflammation) shows in vivo anti-inflammatory activity in Wistar rats.

Key Properties

• CAS Number: 15307-79-6
• MW: 318.13
• Purity: >98% (HPLC)
• Formula: C₁₄H₁₀Cl₂NNaO₂
• SMILES: c1ccc(c(c1)CC(=O)[O-])Nc2c(cccc2Cl)Cl.[Na+]
• Target: COX
• Solubility: Ethanol: 64 mg/mL (201.17 mM); Water: 14 mg/mL (44.0 mM); DMSO: 64 mg/mL (201.17 mM)

Bioactivity

• In Vivo:
  Animal model: Male Sprague-Dawley rats (150±200 g). Dosage: 3 mg/kg. Administration: Oral administration, b. i. d. , for 5 days. Result: Resulted in a significant increase in faecal 51Cr excretion. Animal model: Wistar rats (150-175 g) bearing Formalin-induced rat foot paw edema model. Dosage: 10 mg/kg. Administration: Administered via oral route just prior to induction of inflammation. Result: Showed In vivo anti-inflammatory activity (% edema inhibition = 29.2, 1 h; 22.2, 3 h; 20, 6 h).
• In Vitro:
  Cell Viability Assay Cell line: Neural stem cells (NSCs). Concentration: 1, 3, 10, 30, 60 μM. Incubation time: 1 day. Result: Induction of cell death was concentration-dependent and the effect was not saturated at a concentration of up to 60 μM. Western blot analysis. Cell line: Neural stem cells (NSCs). Concentration: 10, 30 or 60 μM. Incubation time: 6 hours. Result: The activation of caspase-3 was increased in a concentration-dependent manner.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Benfofen | Ecofenac | Voltaren

Quick Database Links

Gene Symbol

COX