Dexamethasone

SKU: orb1309846

Description

Dexamethasone

Research Area

Cell Biology, Immunology & Inflammation, Infectious Disease & Virology, Metabolism Research

Key Properties

• CAS Number: 50-02-2
• MW: 392.46
• Purity: 99.87%
• Formula: C₂₂H₂₉FO₅
• SMILES: C[C@@H]1C[C@H]2[C@@H]3CCC4=CC(=O)C=C[C@]4(C)[C@@]3(F)[C@@H](O)C[C@]2(C)[C@@]1(O)C(=O)CO
• Target: SARS-CoV,ADC Cytotoxin,Antibacterial,Complement System,Antibiotic,Glucocorticoid Receptor,IL Receptor,Mitophagy,Autophagy
• Solubility: DMSO:250 mg/mL (637.01 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:7.3 mg/mL (18.6 mM);Ethanol:6 mg/mL (15.29 mM);H2O:insoluble

Bioactivity

• Target IC50:
  granulocyte-Macrophage cells colony stimulating factor:2.2 nM (EC50)|Fibroblast:> 15 μM|NF-κB, IκBα, and IκBβ:0.5 nM|transcription of the β2-receptor:36 nM (EC50)|COS-7 cells:7.2 nM (EC50)|B-cell:0.8 μM|HeLa S3 cells:4.1 nM|CHO cells:> 20 μM|A549 cells:> 15 μM|Macrophages:0.134 μM|BV-2 cells:2.5 x 10-2 μM|HEK293 cells:> 500 μM|J774 cells:5.2 μM|B16 F10 cells:> 20 μM|HEK293T cells:> 20 μM
• In Vivo:
  METHODS: To investigate the anti-inflammatory effects, Dexamethasone (1-10 mg/kg) was administered as a single intraperitoneal injection to LPS-induced inflammation in C57Bl/6JBom mice. RESULTS: 10 mg/kg Dexamethasone significantly reduced neutrophils in bronchoalveolar lavage fluid.Dexamethasone treatment significantly down-regulated the levels of TNF-α, IL-1α, IL-1β, IL-6, IL-12p40 and MIP-1α mRNA. Dexamethasone exerts anti-inflammatory and antioxidant functions in acute airway inflammation. METHODS: To detect anti-tumor activity in vivo, Dexamethasone (1 mg/kg) was intraperitoneally injected into SCID mice harboring the human myeloma tumor OPM2 five days per week for three weeks. RESULTS: Dexamethasone treatment significantly inhibited the growth of OPM2 tumors, indicating antitumor activity in vivo.
• In Vitro:
  METHODS: Human colorectal cancer cells LoVo and HCT116 were treated with Dexamethasone (10-300 µM) for 72 h. Cell growth inhibition was detected by MTT. RESULTS: Dexamethasone dose-dependently inhibited the growth of LoVo and HCT116 cells, and the inhibition rates of 300 µM Dexamethasone were 52.6% and 58.8%, respectively. METHODS: Acute lymphoblastic leukemia cells RS4;11 were treated with Dexamethasone (100 nM) for 24-36 h. Cell morphology was examined using electron microscopy. RESULTS: In cells treated with Dexamethasone for 24 h, vesicles were surrounded by double membranes, which are characteristic of autophagosomes, and contained membrane structures and/or part of the endoplasmic reticulum or a large amount of cytoplasm. In addition to the appearance of autophagosomes, the nucleus and cell morphology were initially intact, suggesting that autophagosome formation preceded cell death. Dexamethasone induces autophagy. METHODS: Activated mouse and human T cells were treated with Dexamethasone (0.001-10 μM) for 48 h, and PD-1 expression was detected by Flow Cytometry. RESULTS: Dexamethasone enhanced the expression of PD-1 in mouse and human activated T cells.
• Animal Research:
  NAC was administered at three different doses (10, 100 and 500 mg/kg body weight). At the highest concentration, the acidic pH of the NAC solution was adjusted by adding NaOH. Dexamethasone was administered as a single injection of 1 or 10 mg/kg. Both drugs were dissolved in saline and 400 μl were injected intraperitoneally, either 1 h before or 1 h after LPS exposure. In one experiment, NAC (100 and 500 mg/kg) was injected successively every 4·5 h, starting 1 h before challenge (five injections in total). A control group of LPS-exposed animals were injected intraperitoneally with solvent alone (saline). Intratracheal administration was performed by instillation of 100 μl NAC (50, 100 or 500 mg/kg) or dexamethasone (10 mg/kg) into the lungs of mice anaesthetized with 15 mg/kg Rapinovet (i.v.) .

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Dexamethasone, Complement System, ComplementSystem, CD18, CD62L, CD11b, ADC Cytotoxin, ADCCytotoxin, Autophagy, Antibiotic, Bacterial, MK125, MK-125, neutrophils, NSC34521, NSC-34521, Mitophagy, Mitochondrial Autophagy, MK 125, ILReceptor, monocytes, IL Receptor, IL receptor, inhibit, NSC 34521, glucocorticoid, GlucocorticoidReceptor, Glucocorticoid Receptor, Inhibitor, Hexadecadrol, Prednisolone F, receptor, SARSCoV, SARS-CoV, SARS coronavirus

Compound Identifiers

PubChem CID

5743