Desmoglein 1 antibody

SKU: orb2276295

Description

Desmoglein 1 antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

• Tested Applications: IHC
• Dilution Range: 1:75-1:150
• Reactivity: Human
• Application Notes:
  Positive Control: Tonsil: A moderate to strong desmoglein 1 immunostaining should be seen in a fraction of squamous epithelial cells. Negative Control: Desmoglein 1 immunostaining should be absent in all non-squamous epithelial cell types. Cellular Localization: Cell surface Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone No.: MSVA-544M
• Immunogen: Bovine desmoglein (desmosomal glyco protein I) from nasal epidermis.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cadherin family member 4; CDHF4; Desmoglein-1; Desmosomal glycoprotein 1; DG1; DSG1; EPKHE; EPKHIA; Pemphigus foliaceus antigen; PPKS1; SPPK2

Squamous cell carcinoma of the oral cavity with strong Dsg1 positivity of most tumor cells.

Squamous cell carcinoma with variable Dsg1 staining of tumor cells showing only weak staining at the periphery of tumor cell nests.

Squamous epithelium of the skin showing a strong membranous desmoglein 1 immunostaining.

Quick Database Links

UniProt

Q02413