DEL-22379

SKU: orb1300438

Description

DEL-22379

Research Area

Cell Biology, Signal Transduction

Key Properties

• CAS Number: 181223-80-3
• MW: 444.53
• Purity: 99.53%
• Formula: C₂₆H₂₈N₄O₃
• SMILES: COc1ccc2[nH]cc(C=C3C(=O)Nc4ccc(NC(=O)CCN5CCCCC5)cc34)c2c1
• Target: ERK,Apoptosis
• Solubility: DMSO:22.2 mg/mL (49.94 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (4.5 mM)

Bioactivity

• Target IC50:
  ERK:0.5 μM
• In Vivo:
  DEL-22379 (15 mg/kg, i.p.) prevents tumor growth and metastasis in mice bearing A375 (BRAF mutant) and HCT116 (KRAS mutant) tumors, but fails to preven the formation of tumors in mice injected with CHL cells (WT/WT).
• In Vitro:
  DEL-22379 inhibits ERK dimerization without affecting its phosphorylation. In a panel of human cell lines harboring mutant BRAF (V600E) or RAS (Q61L or G12V), DEL-22379 shows potent anti Proliferative effects, and induces apoptosis.
• Cell Research:
  Cellular proliferation is analyzed by Alamar blue assays. Briefly, Cells are plated in 96-well plates at a density of 1000-2000 cells per well. Cells are treated with drug concentration prepared by serial dilution ranging from 0.1 nM to 10 μM. 48 hr after drug treatment cells are exposed to Alamar Blue an the colorimetric change is measured at 570 and 600 nM. GI50 is estimated by nonlinear regression using GraphPad5 Prism Software.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

DEL 22379, DEL22379, DEL-22379, Apoptosis, Inhibitor, inhibit, ERK, Extracellular signal regulated kinases

Compound Identifiers

PubChem CID

69726164