Dasabuvir

SKU: orb1304153

Description

Dasabuvir (ABT-333) is a non-nucleoside inhibitor targeting the HCV NS5B RNA-dependent RNA polymerase, thereby blocking viral replication. It is used in research applications, including in vitro antiviral assays and in vivo studies within combination therapies for hepatitis C, to investigate mechanisms of action and resistance.

Research Area

Cell Biology, Infectious Disease & Virology, Molecular Biology, Protein Biochemistry

Key Properties

• CAS Number: 1132935-63-7
• MW: 493.57
• Purity: 99.62%
• Formula: C₂₆H₂₇N₃O₅S
• SMILES: COc1c(cc(cc1C(C)(C)C)-n1ccc(=O)[nH]c1=O)-c1ccc2cc(NS(C)(=O)=O)ccc2c1
• Target: HCV Protease,DNA/RNA Synthesis
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (4.05 mM);DMSO:60 mg/mL (121.56 mM)

Bioactivity

• Target IC50:
  GnRH:0.33 nM
• In Vitro:
  ABT-333 (Dasabuvir) is at least 7,000-fold selective for the inhibition of HCV genotype 1 polymerases over human/mammalian polymerases. ABT-333 (Dasabuvir) inhibits the polymerase enzymatic activity of genotype 1 laboratory strain enzymes (H77, BK, N, and Con1 strains), as well as enzymes produced from polymerase genes from HCV genotype 1-infected subjects, with IC50s between 2.2 and 10.7 nM. ABT-333 inhibits replication of HCV subgenomic replicons in cell culture assays, with EC50 values of 7.7 and 1.8 nM against genotype 1a (H77) and 1b (Con1), respectively. In the presence of 40% human plasma, there is a 12- to 13-fold decrease in inhibitory potency, yielding EC50s of 99 and 21 nM for HCV genotype 1a (H77) and 1b (Con1) replicons, respectively.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inhibitor, NS5B polymerase, inhibit, genotype, Hepatitis C virus, HCV genotype 1 infections, HCV, HCV Protease, HCV polymerase, HCVProtease, ABT 333, ABT333, ABT-333, DNA/RNA Synthesis, Dasabuvir, RNA

Compound Identifiers

PubChem CID

56640146