DAPI Staining Kit

SKU: orb219885

Description

DAPI (4',6-diamidino-2-phenylindole) is a blue-fluorescent DNA stain that exhibits -20-fold enhancement of fluorescence upon binding to AT regions of dsDNA.It is used extensively in fluorescence microscopy. As DAPI can pass through an intact cell membrane, it can be used to stain both live and fixed cells, though it passes through the membrane less efficiently in live cells and therefore the effectiveness of the stain is lower.

Storage & Handling

• Storage: Store at -20°C for one year.
• Disclaimer: For research use only

Immunofluorescent analysis staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

References

M El Assar, B García-Gómez, JM La Fuente, M Alonso-Isa, JI Martínez-Salamanca Targeting TRPC-5 Channel Inhibition to Improve Penile Vascular Function in Erectile Dysfunction International Journal of Molecular Sciences, (2025)

PubMed:

Huanli Zhao 1, Xuejun Wu 2, Shumeng Yang 3, Lili Jiang 4, Huiying Yu 3, Yubin L Formononetin Alleviates the Inflammatory Response Induced by Carotid Balloon Injury in Rats via the PP2A/MAPK Axis Immunol Invest, (2025)

PubMed: 39985449

Applications

IF

Reactivity

Rat

Fábio Trindade 1, João Almeida-Coelho 1, Cláudia Sousa-Mendes 1, Francisca Saraiva 1, Maria L Arbonés 2 3, Adelino Leite-Moreira 1 4, Rui Vitorino # 1 5 6, Inês Falcão-Pires Myocardial phosphoproteomics unveils a key role of DYRK1A in aortic valve replacement-induced reverse remodelling Basic Res Cardiol, (2025)

PubMed: 40618317

Applications

IF

Reactivity

Human

Zahra Kohan 1, Jalal Shayegh 2, Tohid Kazemi 3, Ehsan Ahmadpour 4, Shabnam Babaei 5, Najibeh Shekari 5 Immune modulation by melanoma-derived exosomes: suppression of BALB/c mice splenic cell proliferation, induction of apoptosis, and cell cycle arrest Mol Biol Rep ., (2025)

PubMed: 40767919

Zhang, Min et al. Neuroprotective effects of miR-30c on rats with cerebral ischemia/reperfusion injury by targeting SOX9 Pathol Res Pract, 216, 153271 (2020)

PubMed: 33161310