DAPI Staining Kit

• Catalog Number: orb219885
• Category: Tools
• Description: DAPI (4',6-diamidino-2-phenylindole) is a blue-fluorescent DNA stain that exhibits -20-fold enhancement of fluorescence upon binding to AT regions of dsDNA.It is used extensively in fluorescence microscopy. As DAPI can pass through an intact cell membrane, it can be used to stain both live and fixed cells, though it passes through the membrane less efficiently in live cells and therefore the effectiveness of the stain is lower.
• Tested applications: ICC, IF
• Storage: Store at -20°C for one year.
• Note: For research use only
• Application notes: DAPI (4,6-diamidino-2-phenylindole) is a cell permeable fluorescent minor groove-binding probe for DNA. It binds to the double-stranded DNA (especially to AT rich DNA), and forming a stable fluoresces complex. DAPI throughout the live and dead cell membrane that can be utilized for DAN detect for chromosome DAN, Yeast DNA, chloroplast DNA, Virus DNA and so on. DAPI-DNA complex shows light blue flourescence color with excitation light 364 nm and emission light 454 nm.Experiment Notes: This product is 1 mg/ml Chromogen. Solute it with suitable density for applies. Recommend density for tissue staining is 1-2 ug/ml (on the 0.5 ug/ml, flourescence become light saturation). For cell culture the density is 0.1 ug/ml.
• Expiration Date: 12 months from date of receipt.

References

Zhang, Min et al. Neuroprotective effects of miR-30c on rats with cerebral ischemia/reperfusion injury by targeting SOX9 Pathol Res Pract, 216, 153271 (2020)

PubMed: 33161310

Immunofluorescent analysis staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).