Dansylcadaverine

SKU: orb1307205

Description

Dansylcadaverine

Research Area

Cell Biology

Key Properties

• CAS Number: 10121-91-2
• MW: 335.46
• Purity: 99.60%
• Formula: C₁₇H₂₅N₃O₂S
• SMILES: CN(C)c1cccc2c(cccc12)S(=O)(=O)NCCCCCN
• Target: Autophagy
• Solubility: DMSO:9 mg/mL (26.83 mM)

Bioactivity

• In Vitro:
  The amount of actin-thymosin β4 crosslink is very low in the presence of Dansylcadaverine and transglutaminase, while there are large amounts of Dansylcadaverine-labeled thymosin β4 and actin. The amount of cross-link further decreases in the presence of Dansylcadaverine competing for Lys residues of either G-actin or thymosin β4 (100 μM). In the absence of thymosin β4, Dansylcadaverine-labeled actin polymerized to F-actin.
• Cell Research:
  Dansylcadaverine staining a. Solution preparation: 1. Mother solution preparation: Prepare a certain concentration of Dansylcadaverine mother solution, store it at -20℃ or -80℃ in the dark after aliquoting. 2. Working solution preparation: Select the appropriate working solution concentration according to the experimental requirements, and try to prepare it before use. b. Operation steps: 1. Inoculate cells in a 6-well plate (or 12-well plate) at a concentration of 3x104 cells/well, and culture in a 5% CO2 incubator at 37°C. 2. Add 50 mM Dansylcadaverine working solution to each well and incubate for 15 minutes. 3. Rinse the cells 3 times with PBS and observe the staining under a microscope.

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Monodansyl cadaverine, autofluorescent, Autophagy, autophagic, Dansylcadaverine, probe, substrate, vacuoles

Compound Identifiers

PubChem CID

4247