D-Luciferin Sodium

SKU: orb1306901

Description

D-Luciferin Sodium

Key Properties

• CAS Number: 103404-75-7
• MW: 302.29
• Purity: 99.91%
• Formula: C₁₁H₇N₂NaO₃S₂
• SMILES: [Na+].Oc1ccc2nc(sc2c1)C1=N[C@H](CS1)C([O-])=O
• Target: Others
• Solubility: H2O:100 mg/mL (330.81 mM);DMSO:16.67 mg/mL (55.15 mM);10% DMSO+40% PEG300+5% Tween-80+45% Saline:1.5 mg/mL (4.96 mM)

Bioactivity

• Cell Research:
  I. Solution preparation 1. Preparation of stock solution: Dissolve D-Luciferin sodium in saline or PBS to prepare a stock solution of appropriate concentration, with a common concentration range of 1-10mM. 2. Preparation of working solution: Dilute the D-Luciferin sodium stock solution to the required working solution concentration (generally 100 μM to 1 mM) II. In vivo and in vitro imaging Experimental steps: 1. Add to cells or animals: According to experimental requirements, inject or add D-Luciferin sodium into cell culture medium, animal body or target cells. For in vivo imaging of animals, it is usually administered by intraperitoneal injection or intravenous injection. 3. Reaction initiation: After D-Luciferin sodium reacts with luciferase, it produces detectable bioluminescence at a specific wavelength (usually around 550 nm). Use a bioluminescence imaging system (BLI) or a fluorescence detector for real-time monitoring. 4. Image acquisition: Use an imaging system to obtain luminescence signals and analyze image data to obtain bioluminescence information about cells, tissues or organs. II. Luciferase activity detection: Experimental steps: 1. Prepare D-Luciferin sodium solution (concentration is generally 100 μM to 1 mM). b. Take an appropriate amount of cell or animal sample labeled with luciferase. c. Add D-Luciferin sodium solution to the sample to start the luminescence reaction. d. Use a bioluminescence detector to record the luminescence signal in real time and perform data analysis. 3. Cell experiment: Experimental steps: a. Cultivate cells with luciferase gene. b. Add an appropriate amount of D-Luciferin sodium solution and incubate until the reaction is complete. c. Detect the luminescence signal by a fluorescence spectrophotometer or bioluminescence imaging device.

Storage & Handling

• Storage: keep away from direct sunlight,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Beetle Luciferin, D Luciferin Sodium, DLuciferin Sodium, D-Luciferin Sodium, D-Luciferin, Firefly luciferin, inhibit, Inhibitor

Compound Identifiers

PubChem CID

2733762