D-Biotin

SKU: orb1224565

Description

Biotin is a water-soluble B-vitamin and is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids.(In Vitro):Biotin exhibits higher affinity on breast cancer (T47D) cells over normal mammary epithelial (MCF-12A) cells, with Kms of 9.24 μM and 53.1 μM, respecetively.Biotin (0.09-100 μM; 0-70 min) is dose-dependently uptake by T47D cells with Vmax of 27.34 pmol/mg protein/min.Biotin (1-1000 nM; 24 h) exerts function in the recovery of 7β-OHC (50 μM)-induced cell death and reduces cell adhesion.(In Vivo):Biotin (15 mg/kg/d; p.o.; 12 d) improves nephrotoxicity in streptozotocin-induced (150 mg/kg; i.p.) diabetic mice.Biotin (0.012 mg/kg/d; p.o.; 70 d) deficiency impaires the immune function of the head kidney, spleen and skin in fish with inadequate administration dose.

Key Properties

• CAS Number: 58-85-5
• MW: 244.31
• Purity: >98% (HPLC)
• Formula: C₁₀H₁₆N₂O₃S
• SMILES: C1C2C(C(S1)CCCCC(=O)O)NC(=O)N2
• Target: Vitamin
• Solubility: DMSO: 49 mg/mL (200.56 mM)

Bioactivity

• In Vivo:
  Biotin (15 mg/kg/d; p.o. ; 12 d) improves nephrotoxicity in streptozotocin-induced (150 mg/kg; i.p.) diabetic mice. Biotin (0.012 mg/kg/d; p.o. ; 70 d) deficiency impaires the immune function of the head kidney, spleen and skin in fish with inadequate administration dose. Animal model: Streptozotocin-induced Diabetes type 1 in male Swiss albino mice (25±2 g). Dosage: 15 mg/kg/d. Administration: Oral gavage; 12 days. Result: Improved histopathological results, including distorted glomeruli, inflammatory cells, and giant macrophages, and diminished acroline reaction of oxidative damage. Animal model: Grass carp (117±0.5 g). Dosage: 0.012, 0.110, 0.214, 0.311, 0.427, and 0.518 mg/kg. Administration: Oral gavage; 70 days. Result: Reduced the activities of lysozyme (LZ) and acid phosphatase (ACP), decreased the contents of complement 3 (C3), C4 and immunoglobulin M (IgM). Reduced the mRNA levels of anti-microbial substances. Increased the mRNA levels of pro-inflammatory cytokines, tumour necrosis factor partially and reduced anti-inflammatory IL-4/13A, IL-10, IL-11 and TGF-β1 mRNA levels partially in association with target of rapamycin (TOR) signaling.
• In Vitro:
  Biotin exhibits higher affinity on breast cancer (T47D) cells over normal mammary epithelial (MCF-12A) cells, with Kms of 9.24 μM and 53.1 μM, respecetively. Biotin (0.09-100 μM; 0-70 min) is dose-dependently uptake by T47D cells with Vmax of 27.34 pmol/mg protein/min. Biotin (1-1000 nM; 24 h) exerts function in the recovery of 7β-OHC (50 μM)-induced cell death and reduces cell adhesion. Cell Viability Assay Cell line: Murine oligodendrocyte 158N cells. Concentration: 1, 10, 100, 1000 nM. Incubation time: 24 hours. Result: Showed cytoprotective effects and prevents 7β-hydroxycholesterol-induced disruption of redox status. Improved attenuation of oxidative stress, mitochondrial dysfunction, lipid metabolism alteration.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

D-Biotin | NSC 63865 | Vitamin B7

Quick Database Links

Gene Symbol

Vitamin