Cytokeratin, Pan antibody

• Catalog Number: orb2276600
• Category: Antibodies
• Description: Cytokeratin, Pan antibody validated for immunohistochemistry on 76 different Normal Tissues
• Clonality: Monoclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• UniProt ID: Q7Z794, Q01546
• Tested applications: IHC
• Dilution range: 1:100-1:200
• Application notes: Positive Control: Liver: A strong staining of all bile duct epithelial cells and an at least moderate, predominantly membranous staining of most hepatocytes should be seen. Negative Control: Tonsil: All lymphocytes must not stain. However, interstitial reticulum cells with dendritic/reticular pattern show a weak to moderate fibrillar staining. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Clone Number: Biorbyt-000R
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: K1B; KRT1B; Keratin, type II cytoskeletal 1b; K77;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Invasive lobular breast cancer showing strong diffuse CKpan positivity in tumor cells.

Liver In the liver a strong cytoplasmic staining of all bile ductal epithelial cells and an at least moderate predominantly membranous immunostaining of hepatocytes is seen.

Tonsil, surface epithelium - Squamous epithelial cells are strongly CKpan positive while all lymphocytes are CKpan negative.