Cytokeratin, Pan antibody

SKU: orb2276600

Description

Cytokeratin, Pan antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

• Tested Applications: IHC
• Dilution range: 1:100-1:200
• Reactivity: Human
• Application Notes:
  Positive Control: Liver: A strong staining of all bile duct epithelial cells and an at least moderate, predominantly membranous staining of most hepatocytes should be seen. Negative Control: Tonsil: All lymphocytes must not stain. However, interstitial reticulum cells with dendritic/reticular pattern show a weak to moderate fibrillar staining. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: Biorbyt-000R

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

K1B; KRT1B; Keratin, type II cytoskeletal 1b; K77; CK-1B; Keratin 1B; Keratin-77; Cytokeratin-1B; Type-II Keratin Kb41

Invasive lobular breast cancer showing strong diffuse CKpan positivity in tumor cells.

Liver In the liver a strong cytoplasmic staining of all bile ductal epithelial cells and an at least moderate predominantly membranous immunostaining of hepatocytes is seen.

Tonsil, surface epithelium - Squamous epithelial cells are strongly CKpan positive while all lymphocytes are CKpan negative.

Quick Database Links

UniProt

Q01546, Q7Z794