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Catalog Number | orb257987 |
---|---|
Category | Proteins |
Description | IL-4 is a pleiotropic cytokine produced by activated Th2 cells and mast cells, and plays a pivotal role in immune responses. The effects of IL-4 are mediated after binding to high affinity receptor complexes present on hematopoietic as well as non-hematopoietic cells. Hematopoietic cellular responses to IL-4 are mediated by a high affinity receptor complex comprised of the 140 kDa IL4Rα (CD124)subunit and the 70 kDa common cytokine γc chain (CD132). Interleukin 4 Receptor (IL4R) also known as CD124, IL4Rα and BSF receptor, is a type I cytokine receptor produced by activated Th2 cells and mast cells, and plays an important role in Th2-biased immune responses, alternative macrophage activation, mucosal immunity, allergic inflammation, tumor progression, and atherogenesis. A soluble form of the encoded IL4R protein can be produced by an alternate splice variant or by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. IL4R can alternatively associate with IL13Ra1 to form the type II receptor which is responsive to both IL4 and IL13. Interleukin-4 receptor has been shown to interact with SHC1. |
Reactivity | Monkey |
Tag | C-Fc |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 50.4 kDa |
Target | IL-4 R alpha |
Source | Cynomolgus / Rhesus macaque IL-4 R alpha, Fc Tag (orb257987) is expressed from human 293 cells (HEK293). It contains AA Met 26 - Arg 232 (Accession # G7Q0S7). |
Expression System | HEK293 |
Biological Activity | Immobilized Human IL-4, premium grade at 5 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque IL-4 R alpha, Fc Tag with a linear range of 10-78 ng/mL (QC tested). |
Expression Region | Met 26 - Arg 232 |
Endotoxins | 1.0 EU per μg |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH7.5 |
Alternative names | IL4R,CD124,IL4RA Read more... |
Note | For research use only |
Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 50.4 kDa. The protein migrates as 60-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Monkey IL-4 R alpha protein
Immobilized Human IL-4, premium grade (Cat. No. orb257598) at 5 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque IL-4 R alpha, Fc Tag (Cat. No. orb257987) with a linear range of 10-78 ng/mL (QC tested).
Unconjugated | |
95% | |
25.6 kDa | |
Cynomolgus / Rhesus macaque IL-4 R alpha, His Tag (orb257988) is expressed from human 293 cells (HEK293). It contains AA Met 26 - Arg 232 (Accession # G7Q0S7). |
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