CW-069

SKU: orb1300978

Description

CW-069 is a potent and selective allosteric inhibitor of the HSET (KIFC1) microtubule motor protein, with an IC50 of 75 µM. It demonstrates high specificity over the related kinesin KSP and is a valuable chemical probe for studying HSET function in cancer cell division and aneuploidy research in vitro.

Research Area

Signal Transduction

Key Properties

• CAS Number: 1594094-64-0
• MW: 500.33
• Purity: 97.52% (May vary between batches)
• Formula: C₂₃H₂₁IN₂O₃
• SMILES: OC(=O)c1cc(I)ccc1NC(=O)[C@H](Cc1ccccc1)NCc1ccccc1
• Target: Microtubule Associated,Kinesin
• Solubility: Ethanol:4 mg/mL (7.99 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:3.3 mg/mL (6.6 mM);DMSO:93 mg/mL (185.88 mM);H2O:< 1 mg/mL (insoluble or slightly soluble)

Bioactivity

• Target IC50:
  HSET:75 μM
• In Vitro:
  CW069 increases multipolar spindles in N1E-115 cells with supernumerary centrosomes without altering bipolar spindle morphology in normal human dermal fibroblast cells. CW069 inhibits growth in cancer N1E-115 cells with IC50 of 10 μM, but not in NHDF or primary human bone marrow cells.
• Cell Research:
  Cells are cultured in DMEM supplemented with 10% fetal calf serum (FCS) at 37°C and 5% CO2. All compounds used in the Sulforhodamine B colorimetric (SRB) assay are dissolved in DMSO and diluted in culture medium to a final concentration of 0.2% DMSO. For the SRB assay and live-cell imaging, cells are seeded in 96-well plates at a density of 2,500 cells per well. After 24 hr, the cells are treated with compound for 72 hr, with triplicate wells for each concentration. For the SRB assay, the cells are then fixed with trichloroacetic acid (TCA) and stained with SRB. Fluorescence is quantified using an Infinite 200 PRO plate-reader at a wavelength of 545 nm. Compound-treated wells are compared with solvent control wells and the concentration of compound that results in 50% of the solvent-control cell growth is designated as the IC50 concentration, calculated using Graphpad PRISM 6. At least three biological replicates are performed for each assay.(Only for Reference)

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inhibitor, HSET, inhibit, Kinesin, Microtubule Associated, MicrotubuleAssociated, CW-069, CW 069, CW069

Compound Identifiers

PubChem CID

73427517