Cucurbitacin E

SKU: orb1301289

Description

Cucurbitacin E

Research Area

Cell Biology

Key Properties

• CAS Number: 18444-66-1
• MW: 556.69
• Purity: 98.88%
• Formula: C₃₂H₄₄O₈
• SMILES: CC(=O)OC(C)(C)\C=C\C(=O)[C@](C)(O)[C@H]1[C@H](O)C[C@@]2(C)[C@@H]3CC=C4[C@@H](C=C(O)C(=O)C4(C)C)[C@]3(C)C(=O)C[C@]12C
• Target: CDK,Autophagy
• Solubility: 10% DMSO+90% Corn Oil:2.5 mg/mL (4.49 mM);DMSO:55 mg/mL (98.8 mM)

Bioactivity

• In Vitro:
  the anti-proliferation and cell cycle G2/M arrest induced by Cucurbitacin E (CuE) in CRC cells. MPM-2 flow cytometry tests show that CuE-treated cells accumulated in metaphase (CuE 2.5-7.5 μM). CuE produced G2/M arrest as well as the downregulation of CDC2 and cyclin B1 expression and dissociation. Both effects increased proportionally with the dose of CuE; however, the inhibition of proliferation, arrest of mitosis, production of reactive oxygen species (ROS), and loss of mitochondrial membrane potential (ΔΨm) were found to be dependent on the quantity of CuE used to treat the cancer cells. In addition, cell cycle arrest in treated cells coincided with the activation of the gene GADD45(α, β, γ). Incubation with CuE resulted in the binding of GADD45γ to CDC2, which suggests that the delay in CuE-induced mitosis is regulated by the overexpression of GADD45γ. CuE may have antitumor activities in established CRC.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NF-κB, p65, IL Receptor, JAK, inhibit, Inhibitor, ERK, Autophagy, Caspase, Cyclin dependent kinase, Cucurbitacin E, CDK, CDK1/cyclinB1, p21, p38 MAPK, TNF-α, ROS, STAT, α-Elaterin, α-Elaterine, VEGFR

Compound Identifiers

PubChem CID

5281319