Crocin-4

SKU: orb3054193

Description

Crocin-4, a carotenoid constituent of saffron, is a potent and brain-penetrant antioxidant agent. Crocin-4 can inhibit the aggregation and the concomitant deposition of Aβ fibrils in the brain.

Key Properties

• CAS Number: 55750-86-2
• MW: 504.576
• Purity: >98% (HPLC)
• Formula: C₂₇H₃₆O₉

Bioactivity

• In Vivo:
  Crocin-4 (50 mg/kg; i.p.) is capable of crossing the Blood Brain Barrier (BBB) and build up levels in the mouse brain.
• In Vitro:
  Crocin-4 (0.1-1000 μM; 24-72 h) do not compromise cell viability of neuron-like cells. Crocin-4 (1 mM; 72 h) reduces total PSEN1, PSEN1 and PSEN2 complexes, BACE1, APP-C99 and sAPPα, while it increases PSEN1-CTF and PSEN2 in SH-SY5Y-APP cells. Crocin-4 (1 mM; 72 h) suppresses GSK3β and ERK1/2 kinases in PC12-htau cells, and significantly reduces the levels and phosphorylation of tau on the pThr231 and pSer199/Ser202 epitopes. Western blot analysis. Cell line: Differentiated SH-SY5Y-APP cells. Concentration: 1 mM. Incubation time: 72 hours. Result: Significantly reduced total PSEN1 by 19%, and the PSEN1 and PSEN2 complexes by 81 and 65%, while it increased PSEN1-CTF (26%) and PSEN2 (43%). The key molecules of the amyloidogenic pathway, BACE1 and APP-C99 were significantly reduced by 20 and 28%, respectively. The non-amyloidogenic pathway product sAPPα was also reduced, by 44%, while APP-C83 remained unaltered. Western blot analysis. Cell line: Differentiated PC12-htau cells. Concentration: 1 mM. Incubation time: 72 hours. Result: Significantly reduced total tau levels (by 32%) and tau phosphorylation (pThr231 and pSer199/Ser202-tau by 22 and 75%, respectively). Downregulates the active and inactive forms of GSK3β (total GSK3β by 34%, pSer9-GSK3β by 30%) and ERK1/2 (total ERK2 by 37%, pERK1 by 40%, pERK2 by 50%).

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only