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Crasp2 Control protein

SKU: orb345972

Description

Crasp2 Control protein

Research Area

Protein Biochemistry

Images & Validation

Tested ApplicationsSDS-PAGE, WB
Application Notes
Crasp2 is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect bands at 67.8 kDa for CRASP-2-MBP, (25.4 kDa for CRASP-2 and 42.4 kDa for MBP) and in size corresponding to Crasp2 by Western blotting in the appropriate cell lysate or extract. Complement Regulator-Acquiring Surface Protein 2 was tested in SDS-page and western blot.

Key Properties

SourceBorrelia burgdorferi
Expression SystemE. coli
Biological OriginBorrelia burgdorferi
TargetcspZ, BB_H06
ConjugationUnconjugated
PurityCrasp2 is a fusion protein with an MBP tag and was expressed in E. coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified Crasp2 and was estimated to be greater than 90% pure.

Storage & Handling

StorageStore vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
Form/AppearanceLiquid (sterile filtered)
Buffer/PreservativesPreservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration1.0mg/mL
Expiration Date6 months from date of receipt.
Dry Ice ShippingPlease note: This product requires shipment on dry ice. A dry ice surcharge will apply.
DisclaimerFor research use only

Alternative Names

control protein, Borrelia burgdorferi CRASP-2, CRASP2
Quality Guarantee

Quality Guarantee

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide
WB
Western Blot (IB, immunoblot)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol