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Catalog Number | orb344684 |
---|---|
Category | Antibodies |
Description | CRASP-2 antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Bacteria |
Isotype | IgG |
Immunogen | MBP-fusion protein corresponding to Borrelia burgdorferi CRASP-2 protein. |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:2,000, WB: 1:1,000 |
Form/Appearance | Lyophilized |
Purity | This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi CRASP-2 protein. A BLAST analysis was used to suggest reactivity with CRASP-2 from B. burgdorferi sources based on 100% homology with the immunizing sequence. Partial cross-reactivity is expected against B. garinii, B. spielmanii, and valaisiana sources based on 91-89% homology. Cross-reactivity with CRASP-2 from other sources has not been determined. |
Conjugation | Unconjugated |
UniProt ID | O50665 |
NCBI | NP_045500.1 |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-CRASP-2 Antibody, Borrelia burgdorferi Read more... |
Note | For research use only |
Application notes | This protein-A purified antibody has been tested for use in ELISA and Western blotting. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 25.4 kDa in size corresponding to Borrelia burgdorferi CRASP-2 protein by Western blotting in the appropriate cell lysate or extract. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of recombinant CRASP-2 protein and Molecular weight markers using CRASP-2 antibody
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