CP-466722

SKU: orb1300927

Description

CP-466722

Research Area

Molecular Biology, Signal Transduction

Key Properties

• CAS Number: 1080622-86-1
• MW: 349.35
• Purity: 99.85% (May vary between batches)
• Formula: C₁₇H₁₅N₇O₂
• SMILES: COc1cc2ncnc(-n3nc(nc3N)-c3ccccn3)c2cc1OC
• Target: ATM/ATR
• Solubility: DMSO:< 1 mg/mL (insoluble or slightly soluble)

Bioactivity

• Target IC50:
  ATM:410 nM
• In Vitro:
  CP-466722 is used as a potential inhibitor to inhibits the activity of ATM kinase, which phosphorylates GST-p53(1–101) substrate. CP-466722 also exhibits the decreasing activities against abl and src kinases. In MCF-7 human breast cancer cells and primary and immortalized diploid human fibroblasts, CP-466722 suppress ATM-dependent p53 induction. In HeLa cells, CP-466722(6 μM), leads to the inhibition in ATM-dependent phosphorylation by reversibly inhibiting ionizing radiation (IR)-induced ATM kinase activity.
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• Cell Research:
  HeLa or A-T (GM02052 expressing hTERT) cells are plated in triplicate and incubated for 24 hours. Cells are pre-treated: DMSO, CP466722 or KU55933 prior to IR (0-10 gy). Cells are incubated for 4 hours following IR before media is removed, cells washed (PBS), trypsined, counted and re-plated (2000 cells/plate, 10 cm plates) in the absence of drug and incubated for 10 days. Prior to colony counting, cells are washed (PBS), stained (PBS, 0.0037%v/v-formaldehyde, 0.1%w/v-crystal violet), rinsed (dWater) and dried. Defined populations (>50 cells) are counted as one surviving colony, data are calculated as percentage surviving colonies relative to control plates +/− SE. (Only for Reference)
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CP466722, CP-466722, CP 466722, ATM/ATR, ATM and RAD3 related, ATM, Ataxia telangiectasia mutated, ATR, Inhibitor, inhibit

Compound Identifiers

PubChem CID

44551660