Concanavalin A

SKU: orb1303318

Description

Concanavalin A

Research Area

Cell Biology, Infectious Disease & Virology

Key Properties

• CAS Number: 11028-71-0
• MW: 104-112 kDa
• Target: Apoptosis,Antibiotic
• Solubility: H2O:soluble;PBS:5 mg/mL

Bioactivity

• Target IC50:
  ML-1 cells:10 μg/ml|CT-26 cells:10 μg/ml|HIV-1:2.2–2.6 μg mL (EC50)|HSV:98 nM|HepG2 cells:5 μg/ml|MCF-7 cells:15 μg/ml|Huh7 cells:20 μg/ml
• In Vivo:
  METHODS: To assay antitumor activity in vivo Concanavalin A (10 mg/kg, intravenously) and ISO-1 (10 mg/kg, intraperitonealy) were administered to BAL-/- mice bearing ML-1 liver tumor nodules twice every three days for 30 days. Results: Concanavalin A treatment significantly reduce the number and size of hepatic tumor nodules and in the presence of ISO-1 the therapeutic effect of Concanavalin A was abolished. MIF does play an important role in Concanavalin A-induced autophagy and antitumor activity in vivo
• In Vitro:
  METHODS: Human hepatocellular carcinoma cells HuH-7 and Hep G2 were treated with Concanavalin A (1-40 µM) for 3-48 h, an the expression levels of target proteins were detected by Western Blot. Results: After Concanavalin A stimulation, LC3-II In both human hepatocellular carcinoma cell lines increased in a dose- and time-dependent manner. METHODS: HUVECs and EA.hy926 cells were treated with Concanavalin A (0.3-1 µg/mL) for 6-24 h the pro-angiogenic activity was detected by Matrigel tube formation and ELISA assay. Results: Different concentration of Concanavalin A, especially at 1 or 3 µg/mL, significantly promoted angiogenesis in HUVEC and Ea.hy926 cells the total Primary fragment length in HUVEC and Ea.hy926 cells was significantly higher in Concanavalin A-treated cells compared to the negative control.

Storage & Handling

• Storage: store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Antibiotic, Apoptosis, Concanavalin A, jack bean, inhibit, glucose, Inhibitor, mannose, plant lectin, programmed cell death

Compound Identifiers

PubChem CID

155486958