Collagen: Strengthening your vital research

Collagen Background

Collagen is the most abundant protein in the human body, accounting for nearly a third of protein composition, with at least 16 different isoforms; it can be observed in nearly all tissues. Collagen plays a vital role as a structural protein in connective tissues such as the tendons, ligaments and skin. It provides tensile strength and elasticity, often working alongside other important proteins such as keratin and elastin. Its abundance and variety in form and function make it an interesting protein worthy of research.

IHC-P image of mouse lymph node tissue using Collagen I antibody (5 ug/ml) - orb322979.

IF analysis of mouse lung tissue using Collagen II antibody (dilution of primary antibody at 1:300) - orb10436.

Collagen Structure

The shape and structural properties of collagen are the result of its triple-helical α-domains. In classical collagen, a single triple-helical domain comprises nearly 95% of the molecule. The correct folding of each of these chains requires a glycine residue at every third position in the polypeptide chain. Each α-chain is composed of multiple triplet sequences of Gly-Y-Z. Y is usually proline and Z is predominantly hydroxyproline. The presence of hydroxyproline at the Y position likely contributes to the stability of collagen. These three α-chains are then twisted around one another in a rope-like manner to produce the tightly packed triple-helical structure of collagen. The interaction of α-chains is stabilized via interchain hydrogen bonding.

Immunohistochemical staining of paraffin embedded rat skin tissue using Collagen III antibody (2.5 ug/ml) - orb322981.

IHC-P image of rat colon tissue using Collagen IV antibody (2.5 ug/ml) - orb340147.

Collagen Function

Type	Function	Associated Disease
Collagen I	Accounts for 90% of your body's collagen, made of densely packed fibres. It provides structure to skin, bones, tendons, fibrous cartilage, connective tissue and teeth.	Osteogenesis imperfecta (Brittle Bone Disease) - A disorder that results in fragile bones that break easily. It's typically present at birth.
Collagen II	Made from more loosely packed fibres, it is found in elastic cartilage, which cushions joints.	Chondrodysplasias - A group of conditions that affect skeletal development and growth. The conditions are often linked to dwarfism, which stunts the growth of many parts of the body, particularly the bones.
Collagen III	Secreted by fibroblasts and other mesenchymal cell types, making it a major player in a variety of inflammation-driven pathologies such as lung injury, viral and nonviral liver diseases.	Atopic Dermatitis - The most common type of eczema, a condition that makes the skin red and itchy.
Collagen IV	The main collagen type found in the basement membrane. It is a network-forming collagen that underlies epithelial and endothelial cells, functioning as a barrier between tissue compartments.	Ehlers-Danlos Syndrome - A group of conditions that cause very flexible joints and stretchy and fragile skin.

Biorbyt has a highly validated and comprehensive range of Collagen proteins, antibodies and genes including the difficult to source Gallus Collagen I and Goldfish Collagen 1 and Guinea Pig Elastin antibodies. We also offer the hard to find Bovine Collagen I antibody.

IF analysis of rat stomach tissue using Collagen 4 alpha 1 antibody (2.5 ug/ml) - orb382133.

IHC-P staining of rat liver tissue using Collagen X antibody (2.5 ug/ml) - orb10444.

Why not look through and refine your search for our whole Collagen range. Our favourite and most unique products are highlighted below:

Monoclonal Antibodies

Catalog #	Target	Host	Reactivity
orb653618	Collagen I	Mouse	Human
orb653619	Collagen III	Mouse	Human
orb653620	Collagen IV	Mouse	Human

Polyclonal Antibodies

Catalog #	Target	Host	Reactivity
orb653621	Collagen I	Rabbit	Human
orb653622	CO1A2	Rabbit	Human
orb653623	Collagen III	Rabbit	Human
orb653624	Collagen IV	Rabbit	Human
orb653625	Collagen V	Rabbit	Human
orb653626	Collagen VI	Rabbit	Human
orb653627	Endostatin	Rabbit	Human
orb653628	Fibronectin	Rabbit	Human
orb653629	Elastin	Rabbit	Human
orb653630	Elastin	Guinea Pig	Human
orb653631	Collagen I	Rabbit	Bovine
orb653632	CO1A2	Rabbit	Bovine
orb653633	Collagen III	Rabbit	Bovine
orb653634	Collagen IV	Rabbit	Bovine
orb653635	Collagen I	Rabbit	Gallus
orb653636	CO1A2	Rabbit	Gallus
orb653637	Collagen III	Rabbit	Gallus
orb653653	Collagen I	Rabbit	Fish
orb653635	Collagen I	Rabbit	Gallus

ELISA Kits

Catalog #	Target	Reactivity
orb404181	Collagen I	Human
orb404168	Collagen II	Human
orb405759	Collagen III	Human
orb405761	Collagen IV	Human
orb404197	Collagen V	Human
orb407920	Collagen I	Mouse
orb408788	Collagen II	Mouse
orb407905	Collagen III	Mouse
orb407906	Collagen IV	Mouse
orb408872	Collagen V	Mouse

Peptides

Catalog #	Target	Reactivity	Applications
orb234441	Collagen I	Canine, Human, Mouse, Rabbit	Blocking
orb217115	Collagen III	Canine, Human, Mouse, Rat	-
orb382422	Collagen IV	Human, Mouse, Rat	Blocking
orb382339	ITGA2	Human, Mouse	Blocking
orb382398	COL25A1	Human, Mouse	Blocking
orb382404	COL28A1	Human, Mouse	Blocking
orb382420	COL17A1	Human, Mouse	Blocking
orb218660	COL18A1	Canine, Human, Rat	-
orb218737	CCBE1	Human, Mouse, Rat	-
orb217121	COL11A1	Human, Mouse, Rat	-

Proteins

Catalog #	Target	Reactivity	Applications
orb108488	Collagen I	Bovine	ELISA
orb98979	Collagen III	Human	ELISA, IHC-P, RIA, WB
orb98980	Collagen IV	Human	ELISA, IHC-P, RIA, WB
orb98981	Collagen V	Human	ELISA, IHC-P, RIA, WB
orb391502	Collagen III	Mouse	ELISA, SDS-PAGE, WB
orb82633	Adiponectin Flag tag	Human	ELISA, SDS-PAGE, WB
orb86247	Laminin 5	Human	WB
orb180167	Adiponectin	Mouse	SDS-PAGE
orb180234	CTHRC1	Human	SDS-PAGE
orb311698	COL3A1 (C-His)	Mouse	SDS-PAGE