Collagen

Collagen: Strengthening your vital research

Collagen Background

Collagen is the most abundant protein in the human body, accounting for nearly a third of protein composition, with at least 16 different isoforms; it can be observed in nearly all tissues. Collagen plays a vital role as a structural protein in connective tissues such as the tendons, ligaments and skin. It provides tensile strength and elasticity, often working alongside other important proteins such as keratin and elastin. Its abundance and variety in form and function make it an interesting protein worthy of research.


IHC-P image of mouse lymph node tissue using Collagen I antibody (5 ug/ml)

IHC-P image of mouse lymph node tissue using Collagen I antibody (5 ug/ml) - orb322979.

IF analysis of mouse lung tissue using Collagen II antibody (dilution of primary antibody at 1:300)

IF analysis of mouse lung tissue using Collagen II antibody (dilution of primary antibody at 1:300) - orb10436.

Collagen Structure

The shape and structural properties of collagen are the result of its triple-helical α-domains. In classical collagen, a single triple-helical domain comprises nearly 95% of the molecule. The correct folding of each of these chains requires a glycine residue at every third position in the polypeptide chain. Each α-chain is composed of multiple triplet sequences of Gly-Y-Z. Y is usually proline and Z is predominantly hydroxyproline. The presence of hydroxyproline at the Y position likely contributes to the stability of collagen. These three α-chains are then twisted around one another in a rope-like manner to produce the tightly packed triple-helical structure of collagen. The interaction of α-chains is stabilized via interchain hydrogen bonding.

Immunohistochemical staining of paraffin embedded rat skin tissue using Collagen III antibody (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded rat skin tissue using Collagen III antibody (2.5 ug/ml) - orb322981.

IHC-P image of rat colon tissue using Collagen IV antibody (2.5 ug/ml)

IHC-P image of rat colon tissue using Collagen IV antibody (2.5 ug/ml) - orb340147.

Collagen Function

TypeFunctionAssociated Disease
Collagen I Accounts for 90% of your body’s collagen, made of densely packed fibres. It provides structure to skin, bones, tendons, fibrous cartilage, connective tissue and teeth. Osteogenesis imperfecta (Brittle Bone Disease) - A disorder that results in fragile bones that break easily. It's typically present at birth.
Collagen II Made from more loosely packed fibres, it is found in elastic cartilage, which cushions joints. Chondrodysplasias - A group of conditions that affect skeletal development and growth. The conditions are often linked to dwarfism, which stunts the growth of many parts of the body, particularly the bones.
Collagen III Secreted by fibroblasts and other mesenchymal cell types, making it a major player in a variety of inflammation-driven pathologies such as lung injury, viral and nonviral liver diseases. Atopic Dermatitis - The most common type of eczema, a condition that makes the skin red and itchy.
Collagen IV The main collagen type found in the basement membrane. It is a network-forming collagen that underlies epithelial and endothelial cells, functioning as a barrier between tissue compartments. Ehlers-Danlos Syndrome - A group of conditions that cause very flexible joints and stretchy and fragile skin.

Biorbyt has a highly validated and comprehensive range of Collagen proteins, antibodies and genes including the difficult to source Gallus Collagen I and Goldfish Collagen 1 and Guinea Pig Elastin antibodies. We also offer the hard to find Bovine Collagen I antibody.

IF analysis of rat stomach tissue using Collagen 4 alpha 1 antibody (2.5 ug/ml)

IF analysis of rat stomach tissue using Collagen 4 alpha 1 antibody (2.5 ug/ml) - orb382133.

IHC-P staining of rat liver tissue using Collagen X antibody (2.5 ug/ml)

IHC-P staining of rat liver tissue using Collagen X antibody (2.5 ug/ml) - orb10444.

Why not look through and refine your search for our whole Collagen range. Our favourite and most unique products are highlighted below:

Monoclonal Antibodies

Catalog #TargetHostReactivity
orb653618 Collagen I Mouse Human
orb653619 Collagen III Mouse Human
orb653620 Collagen IV Mouse Human

Polyclonal Antibodies

Catalog #TargetHostReactivity
orb653621 Collagen I Rabbit Human
orb653622 CO1A2 Rabbit Human
orb653623 Collagen III Rabbit Human
orb653624 Collagen IV Rabbit Human
orb653625 Collagen V Rabbit Human
orb653626 Collagen VI Rabbit Human
orb653627 Endostatin Rabbit Human
orb653628 Fibronectin Rabbit Human
orb653629 Elastin Rabbit Human
orb653630 Elastin Guinea Pig Human
orb653631 Collagen I Rabbit Bovine
orb653632 CO1A2 Rabbit Bovine
orb653633 Collagen III Rabbit Bovine
orb653634 Collagen IV Rabbit Bovine
orb653635 Collagen I Rabbit Gallus
orb653636 CO1A2 Rabbit Gallus
orb653637 Collagen III Rabbit Gallus
orb653653 Collagen I Rabbit Fish
orb653635 Collagen I Rabbit Gallus

ELISA Kits

Catalog #TargetReactivity
orb404181 Collagen I Human
orb404168 Collagen II Human
orb405759 Collagen III Human
orb405761 Collagen IV Human
orb404197 Collagen V Human
orb407920 Collagen I Mouse
orb408788 Collagen II Mouse
orb407905 Collagen III Mouse
orb407906 Collagen IV Mouse
orb408872 Collagen V Mouse

Peptides

Catalog #TargetReactivityApplications
orb234441 Collagen I Canine, Human, Mouse, Rabbit Blocking
orb217115 Collagen III Canine, Human, Mouse, Rat -
orb382422 Collagen IV Human, Mouse, Rat Blocking
orb382339 ITGA2 Human, Mouse Blocking
orb382398 COL25A1 Human, Mouse Blocking
orb382404 COL28A1 Human, Mouse Blocking
orb382420 COL17A1 Human, Mouse Blocking
orb218660 COL18A1 Canine, Human, Rat -
orb218737 CCBE1 Human, Mouse, Rat -
orb217121 COL11A1 Human, Mouse, Rat -

Proteins

Catalog #TargetReactivityApplications
orb108488 Collagen I Bovine ELISA
orb98979 Collagen III Human ELISA, IHC-P, RIA, WB
orb98980 Collagen IV Human ELISA, IHC-P, RIA, WB
orb98981 Collagen V Human ELISA, IHC-P, RIA, WB
orb391502 Collagen III Mouse ELISA, SDS-PAGE, WB
orb82633 Adiponectin Flag tag Human ELISA, SDS-PAGE, WB
orb86247 Laminin 5 Human WB
orb180167 Adiponectin Mouse SDS-PAGE
orb180234 CTHRC1 Human SDS-PAGE
orb311698 COL3A1 (C-His) Mouse SDS-PAGE