COL1A1/Collagen I Antibody

• Catalog Number: orb1294293
• Category: Antibodies
• Description: Rabbit polyclonal antibody to COL1A1
• Target: COL1A1 / Collagen I
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit-IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide / encompassing a sequence within the C-terminus region.
• Tested applications: IF, IHC, WB
• Dilution range: Western Blot 1:1000-1:1500 Immunofluorescence 1:300–1:500 Immunohistochemistry (Paraffin) 1:100–1:300
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-COL1A1 / Collagen I antibody - at 1/1000 dilution. Lysates at 50 µg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked before being incubated with orb1294293 overnight at 4°C.

Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min. Cells were stained with orb1294293 anti-COL1A1 / Collagen I antibody (red) at 1:400 and 4°C.

COL1A1 / Collagen I antibody - at 1/1000 dilution. Lysates at 40 µg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked before being incubated with orb1294293 overnight at 4°C.

COL1A1 / Collagen I antibody - at 1/250 dilution. Lysates at 50 µg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked before being incubated with orb1294293 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded mouse testis labeling COL1A1 / Collagen I with orb1294293 at 1/150.

Immunohistochemical analysis of paraffin embedded mouse testis labeling COL1A1 / Collagen I with orb1294293 at 1/150.

Immunohistochemical analysis of paraffin embedded Mouse tissue labeling COL1A1 / Collagen I with orb1294293 at 1/100.

Immunohistochemical analysis of paraffin embedded Mouse tissue labeling COL1A1 / Collagen I with orb1294293 at 1/100.