Coelenterazine

SKU: orb1300448

Description

Coelenterazine

Research Area

Immunology & Inflammation, Metabolism Research, Signal Transduction

Key Properties

• CAS Number: 55779-48-1
• MW: 423.46
• Purity: 99.02%
• Formula: C₂₆H₂₁N₃O₃
• SMILES: Oc1ccc(Cc2nc3c(Cc4ccccc4)[nH]c(cn3c2=O)-c2ccc(O)cc2)cc1
• Target: Others,Reactive Oxygen Species,ROS
• Solubility: Ethanol:1 mg/mL (2.36 mM);H2O:< 1 mg/mL (insoluble or slightly soluble)

Bioactivity

• Target IC50:
  superoxide anion:69 μM|prostate cancer cell:24.28 μM|breast cancer cell:21.56 μM
• In Vivo:
  METHODS: Gli36-Gluc and Gli36-Rluc cells were injected into the leg muscles of nude mice. Mice were injected intravenously with 100 μg (4 mg/kg body weight) of coelenterazine and immediately imaged using a cooled charge-coupled device (CCD) camera. The mice were injected with 500 μg (20 mg/kg) of water-soluble coelenterazine and imaged using a CCD camera. RESULTS Water-soluble coelenterazine produced 10-fold higher photon counts for Gluc and 4-fold higher photon counts for Rluc, indicating that s-coelenterazine is more sensitive for detecting coelenteric acid luciferase in vivo.
• In Vitro:
  When monitoring various biological processes, coelenterazine (CTZ) can be catalyzed by luciferases, such as the sea pansy Renilla reniformis (Rluc) and the marine copepod Gaussia princeps (Gluc), which results in emission of blue light. In Gli36-Gluc- or Gli36-Rluc-expressing Gli36 cells, coelenterazine (8 礸/ml) shows a bioluminescence signal. In RIN-5F, 4T1, HCT 116, HEK 293, 293FT, HeLa and INS-1 cells, coelenterazine can be used to assay superoxide anion concentrations.
• Cell Research:
  Cells are plated at a density of 5×10^4 cells per well into 24-well plates and grown to 80-100% confluency. Just before imaging, media are changed to a colorless solution containing (in mM): 2.7 KCl, 139 NaCl, 8.1 Na2HPO4, 0.7 H2O, 1.5 KH2PO4, 1.8 CaCl2, 1 MgCl2 and 5.5 d-glucose. Cells are preincubated for 15 min in the absence or presence of Pgp modulator, after which Coelenterazine (final concentration of 470 nM) is added directly to the cells. They are for reference only.
• Animal Research:
  Mice are anesthetized with metofane or isoflurane before tail vein injection of Coelenterazine (4 μg/g) formulated from an ethanol stock diluted in sodium phosphate buffer (50 mM). Bioluminescence imaging is performed on the in vivo imaging system at 2, 6, 8, and 11 min after injection. Anesthesia is maintained during imaging by nose cone delivery of 2.5% isoflurane. After imaging, animals are killed by cervical dislocation; tumors are then harvested and weighed. They are for reference only.

Storage & Handling

• Storage: keep away from direct sunlight,store at low temperature | Powder: -20°C for 3 years | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Coelenteramine, Coelenterazine, CTZ, Inhibitor, inhibit, Oplophorus luciferin

Compound Identifiers

PubChem CID

149358008