【抗体精选】文献同款：Histone H3 抗体

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今日推荐文献同款：Histone H3 Antibody（orb10805）——可靠的核内参工具。

今日推荐 · 文献同款

Histone H3 Antibody

一、组蛋白 H3：染色质结构与表观遗传的基石

组蛋白 H3 是一个小分子量（约 15 kDa）的碱性蛋白。与组蛋白 H4 形成四聚体，两个 H3-H4 四聚体进一步与两对 H2A-H2B 二聚体组装成核小体八聚体核心，是染色质高级结构形成的基石。其 N 端尾部及球状结构域上的特定氨基酸残基是多种翻译后修饰的底物，这些修饰构成了表观遗传调控的核心机制。

组蛋白 H3 本身不直接传导信号，但其修饰状态是众多信号通路的终点效应器和调控节点。

► 生长因子 / RAS / MAPK 通路

活化后的激酶（如 MSK1/2、RSK）直接磷酸化 H3S10 和 H3S28，快速诱导即刻早期基因（如 c-Fos）表达，连接胞外刺激与核内转录。

► 细胞周期调控通路

Aurora B 激酶在 G2/M 期磷酸化 H3S10 和 H3S28，是染色体凝集和分离的必要步骤。

► DNA 损伤应答通路

DNA 双链断裂后，ATM/ATR 激酶被激活，进而磷酸化下游效应物，导致 H2AX 磷酸化（γH2AX），并伴随局部 H3K9ac 减少和 H3K9me3 增加，以招募修复蛋白并改变染色质状态。

► Wnt / β-catenin 通路

通路激活后，β-catenin 入核，可招募组蛋白乙酰转移酶（如 CBP/p300）至靶基因启动子，催化 H3K27ac，激活 Wnt 靶基因转录。

二、当前研究热点与前沿

• H3K27M 突变是驱动儿童弥漫性内生性脑桥胶质瘤的核心病因。H3K27M 突变全局性降低 H3K27me3 水平，导致发育基因异常表达。针对此突变的表观遗传疗法（如 EZH2 抑制剂）是研究热点。
• 新型代谢相关修饰的发现与功能：如乳酸化（Lactylation）、组胺化（Histaminylation）、修饰动态平衡的精密调控机制、组蛋白变体的特异性调控等。

在实验科学中，由于组蛋白 H3 在所有有核细胞中恒定、高丰度表达且严格定位于细胞核，组蛋白 H3 抗体被广泛用作 Western Blot 等实验中的核蛋白内参对照，是研究核内事件（如基因转录调控、DNA 损伤修复、核内信号转导）的关键质量控制工具。

三、产品信息

产品	货号	应用
Histone H3 antibody	orb10805	FC, IF, IHC-Fr, IHC-P, WB

四、产品特色与核心优势

Histone H3 兔源多克隆抗体（orb10805）是一款经过充分验证的高通用性核心工具，其核心定位为可靠的「核内参」。该抗体在 WB、IHC、IF 及流式细胞术等多种平台中均表现出优异的性能，并已在人、小鼠、大鼠的多种组织（包括石蜡与冰冻切片）与疾病模型中得到广泛验证。产品本身具备高纯度与高浓度的优势，确保了出色的性价比。其跨物种适用性进一步获得了同行评议文献的支持，相关研究已成功将其应用于大鼠及拟南芥的 WB 实验中，证实了其在更广泛研究领域的实用价值。Histone H3 兔源多克隆抗体（orb10805）是一款数据扎实、应用场景全面的高质量研究试剂。

1. 广泛而严谨的验证体系，确保结果可靠性

• 除常规细胞系（如 K562）外，该抗体已在人、小鼠、大鼠的十余种器官（如脑、肝、肾、睾丸、肠等）以及多种癌症组织（如宫颈癌、结肠癌、喉癌）中成功应用，提供了极为丰富的阳性参照数据。
• 切片类型兼容性佳，经验证适用于石蜡包埋切片（IHC-P）和冰冻切片（IHC-Fr）。
• 在 Western Blot、免疫组化、免疫荧光、流式细胞术中的表现均有具体数据支撑，其识别位点在变性、固定等多种条件下均能稳定暴露并被识别。

Histone H3 Rabbit Polyclonal Antibody (Nuclear Loading Control)

Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HIST3H3) Polyclonal Antibody, Unconjugated (orb10805) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Histone H3 Rabbit Polyclonal Antibody (Nuclear Loading Control)

Paraformaldehyde-fixed, paraffin embedded (human colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (orb10805) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Histone H3 Rabbit Polyclonal Antibody (Nuclear Loading Control)

Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (orb10805) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Histone H3 Rabbit Polyclonal Antibody (Nuclear Loading Control)

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (orb10805) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Histone H3 Rabbit Polyclonal Antibody (Nuclear Loading Control)

Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (orb10805) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Histone H3 Rabbit Polyclonal Antibody (Nuclear Loading Control)

Paraformaldehyde-fixed, paraffin embedded (mouse liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Histone H3 / HIST3H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (orb10805) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Histone H3 Rabbit Polyclonal Antibody (Nuclear Loading Control)

Paraformaldehyde-fixed, paraffin embedded (mouse pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (orb10805) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

2. 详尽的「即用型」方案，极大降低优化成本

• 提供产品验证图片的同时提供了详尽的实验流程，涵盖固定、通透、抗原修复、封闭、抗体孵育等关键步骤的具体条件（如缓冲液配方、温度、时间），帮助您有效缩短实验启动和优化周期。
• 针对不同技术，给出了推荐的起始稀释度范围（如 WB 可达 1:5000–1:50000），并明确了在不同检测系统（DAB 显色或荧光标记）下的配套方案，实用性强。

Histone H3 Rabbit Polyclonal Antibody (Nuclear Loading Control)

Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (orb10805) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Histone H3 Rabbit Polyclonal Antibody (Nuclear Loading Control)

Paraformaldehyde-fixed, paraffin embedded (mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (orb10805) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Histone H3 Rabbit Polyclonal Antibody (Nuclear Loading Control)

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Histone H3 / HIST3H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (orb10805) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Histone H3 Rabbit Polyclonal Antibody (Nuclear Loading Control)

Paraformaldehyde-fixed, paraffin embedded (rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (orb10805) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Histone H3 Rabbit Polyclonal Antibody (Nuclear Loading Control)

Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (orb10805) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Histone H3 Rabbit Polyclonal Antibody (Nuclear Loading Control)

Paraformaldehyde-fixed, paraffin embedded (rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Histone H3 / HIST3H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (orb10805) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Histone H3 Rabbit Polyclonal Antibody (Nuclear Loading Control)

Paraformaldehyde-fixed, paraffin embedded (rat pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (orb10805) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Histone H3 Rabbit Polyclonal Antibody (Nuclear Loading Control)

Paraformaldehyde-fixed, paraffin embedded (rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Histone H3 / HIST3H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (orb10805) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

3. 高质量产品保障与灵活性

• 抗体经 Protein A 亲和纯化，纯度有保障；1 mg/ml 的高浓度原液使其稀释使用成本极低，经济高效。
• 提供多种直接偶联物（如 HRP、FITC、PE、Cy 系列等）可选，方便用户进行多重标记或直接检测，提升了实验设计的灵活性。

Histone H3 Rabbit Polyclonal Antibody (Nuclear Loading Control)

Paraformaldehyde-fixed, paraffin embedded (rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (orb10805) at 1:500 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (YF488) for 90 minutes, and DAPI for nuclei staining.

Histone H3 Rabbit Polyclonal Antibody (Nuclear Loading Control)

Paraformaldehyde-fixed, paraffin embedded (rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (orb10805) at 1:300 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (YF488) for 90 minutes, and DAPI for nuclei staining.

Histone H3 Rabbit Polyclonal Antibody (Nuclear Loading Control)

Paraformaldehyde-fixed, paraffin embedded (mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (orb10805) at 1:300 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (YF488) for 90 minutes, and DAPI for nuclei staining.

Histone H3 Rabbit Polyclonal Antibody (Nuclear Loading Control)

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (orb10805) at 1:300 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (YF488) for 90 minutes, and DAPI for nuclei staining.

Histone H3 Rabbit Polyclonal Antibody (Nuclear Loading Control)

Paraformaldehyde-fixed, paraffin embedded (human liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (orb10805) at 1:300 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (YF488) for 90 minutes, and DAPI for nuclei staining.

Histone H3 Rabbit Polyclonal Antibody (Nuclear Loading Control)

Blank control: K562. Primary Antibody (green line): Rabbit Anti-Histone H3/HIST3H3 antibody (orb10805), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, Dilution: 1 µg/Test. Protocol: The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Histone H3 Rabbit Polyclonal Antibody (Nuclear Loading Control)

Blank control: Mouse spleen cells (blue). Primary Antibody: Rabbit Anti-Histone H3/HIST3H3 antibody (orb10805), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1X PBS containing 0.5% BSA. Protocol: The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (orb10805, 1 µg/1×10^6 cells) were incubated for 30 min on ice, followed by 1X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed.

4. 获得跨学科研究的文献支持

该抗体的有效性已获得独立发表的同行评议文献的支持。不仅在经典的哺乳动物模型（大鼠）研究中得到应用，更在植物学模型（拟南芥）的 WB 实验中得到验证，这强有力地证明了其识别表位的高度保守性和卓越的跨物种反应能力，拓宽了其应用边界。

值得注意的是：Histone H3 抗体作为优秀的核内参，其应用有严格场景，它仅适用于核蛋白提取物。如果实验使用全细胞裂解液，则应选用 GAPDH、β-Actin 等胞质 / 总蛋白内参。

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