Clodronic acid disodium salt

SKU: orb1300758

Description

Clodronate disodium salt is a bisphosphonate compound that potently inhibits osteoclast-mediated bone resorption. It is widely used in osteoporosis research and in vivo models to study bone metabolism, osteolytic diseases, and macrophage depletion.

Research Area

Cell Biology

Key Properties

• CAS Number: 22560-50-5
• MW: 288.86
• Purity: >99.99% (May vary between batches)
• Formula: CH₄Cl₂O₆P₀·2Na
• SMILES: [Na+].[Na+].OP(O)(=O)C(Cl)(Cl)P([O-])([O-])=O
• Target: Others,Apoptosis
• Solubility: H2O:58.82 mg/mL (203.63 mM);DMSO:Insoluble

Bioactivity

• In Vitro:
  Clodronate significantly decreases total viability of cultures of J774 cells with EC50 of 300 μM, while liposome-encapsulated Clodronate decreases total viability of cultures of J774 cells with EC50 of 1 μM. Clodronate and liposome-encapsulated Clodronate is metabolized to a nonhydrolyzable adenosine triphosphate (ATP) analog, adenosine 5'-(beta, gamma-dichloromethylene) triphosphate, which can be detected in J774 cell extracts by using fast protein liquid chromatography. Clodronate induces apoptosis in isolated osteoclasts. Clodronate, when administered in liposomes, also induces apoptosis in rat peritoneal macrophages in vitro and in liver macrophages of mice in vivo but not in murine macrophage-like RAW-264 cells. Clodronate delivered into macrophages by liposome will kill these cells as a result of intracellular accumulation and irreversible metabolic damage. Clodronate encapsulated in liposomes (clodrolip) efficiently depletes the phagocytic cells in the murine F9 teratocarcinoma and human A673 rhabdomyosarcoma mouse tumour models resulting in significant inhibition of tumour growth ranging from 75 to >92%, depending on therapy and schedule. Clodronate, a bisphosphonate that lacks a nitrogen, does not inhibit protein isoprenylation but can be metabolized intracellularly to a beta-gamma-methylene (AppCp-type) analog of ATP, which is cytotoxic to macrophages in vitro. Clodronate is metabolited to AppCCl(2)p, and AppCCl(2)p inhibits mitochondrial oxygen consumption by a mechanism that involves competitive inhibition of the ADP/ATP translocase.

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

osteoclastic, metastases, mitochondria, Paget's, first-generation, cytochrome, Clodronate, Clodronate Disodium, Clodronic acid, Clodronic acid disodium, Clodronic acid disodium salt, resorption

Compound Identifiers

PubChem CID

73554303