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Catalog Number | orb1478127 |
---|---|
Category | Proteins |
Description | Recombinant Macaca fascicularis Claudin(CLDN18)-VLPs (Active) |
Tag | N-terminal 6xHis-tagged (This tag can be tested only under denaturing conditions) |
Form/Appearance | Lyophilized powder |
Buffer/Preservatives | Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4 |
Protein Sequence | MAVTACQGLGFVVSLIGIAGIIAATCMDQWSTQDLYNNPVTAVFNYQGLWRSCVRESSGFTECRGYFTLLGLPAMLQAVRALMIVGIVLGAIGLLVSIFALKCIRIGSMEDSAKANMTLTSGIMFIVSGLCAIAGVSVFANMLVTNFWMSTANMYTGMGGMVQTVQTRYTFGAALFVGWVAGGLTLIGGVMMCIACRGLAPEETNYKAVSYHASGHSVAYKPGGFKASTGFGSNTKNKKTYDGGAHTEDELQSYPSKHDYV |
Protein Length | Full Length |
UniProt ID | A0A2K5VV62 |
MW | 28.7 kDa |
Application notes | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL.Aliquot for long-term storage at -80℃. Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing. |
Endotoxins | Less than 1.0 EU/ug as determined by LAL method. |
Source | Mammalian cell |
Biological Origin | Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) |
Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis CLDN18 at 5 μg/ml can bind Anti-CLDN18.2 recombinant Antibody, the EC50 is 2.806-3.641 ng/ml. |
Expression Region | 1-261aa |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Alternative names | Claudin |
Note | For research use only |
Detected by Mouse anti-6*His monoclonal antibody.
Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis CLDN18 at 5 μg/ml can bind Anti-CLDN18.2 recombinant Antibody, the EC50 is 2.806-3.641 ng/ml.
The purity of VLPs was greater than 90% as determined by SEC-HPLC