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Clatherin Light Chain Antibody (LCA/LCB)
Catalog Number: orb386041
| Catalog Number | orb386041 |
|---|---|
| Category | Antibodies |
| Description | Recognizes proteins of 31-44kDa, which are identified as Clathrin Light Chains (both A & B). Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin light chain regulates the self-assembly of triskelions onto intracellular membranes. Clathrin light chain subunits (LCA and LCB) contribute to regulation of coated vesicle formation to sort proteins during receptor-mediated endocytosis and organelle biogenesis. LCA and LCB are encoded by two discrete genes. They share only 60% homology, and have certain features in common. Both LCA and LCB undergo alternative mRNA splicing, which results in the generation of tissue-specific isoforms. |
| Clonality | Monoclonal |
| Species/Host | Mouse |
| Isotype | Mouse IgG2b, kappa |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
| Purity | Protein G affinity chromatography |
| Immunogen | A recombinant N-terminal protein fragment from human Clathrin light chain was used as the immunogen for the Clathrin Light Chain antibody. |
| UniProt ID | P09497, P09496 |
| Tested applications | IHC-P |
| Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT |
| Application notes | Optimal dilution of the Clathrin Light Chain antibody should be determined by the researcher. |
| Antibody Type | Primary Antibody |
| Clone Number | SPM174 |
| Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
| Storage | Store the Clathrin Light Chain antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
| Hazard Information | This Clathrin Light Chain antibody is available for research use only. |
| Note | For research use only |
IHC testing of FFPE human pancreas with Clathrin Light Chain antibody (clone SPM174). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min.
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