Cladribine

SKU: orb1305319

Description

Cladribine (2CdA) is a purine nucleoside analog that acts as an adenosine deaminase inhibitor, used in research on lymphoproliferative disorders. It is applied in both in vitro and in vivo studies to investigate its cytotoxic mechanisms against malignant lymphocytes, such as in hairy cell leukemia.

Research Area

Cell Biology, Metabolism Research, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 4291-63-8
• MW: 285.69
• Purity: 100.00% (May vary between batches)
• Formula: C₁₀H₁₂ClN₅O₃
• SMILES: Nc1nc(Cl)nc2n(cnc12)[C@H]1C[C@H](O)[C@@H](CO)O1
• Target: Adenosine Deaminase|||Apoptosis
• Solubility: Ethanol:< 1 mg/mL (insoluble or slightly soluble);DMSO:255 mg/mL (892.58 mM);H2O:< 1 mg/mL (insoluble or slightly soluble);10% DMSO+40% PEG300+5% Tween 80+45% Saline:5 mg/mL (17.5 mM)

Bioactivity

• Target IC50:
  Adenosine deaminase (U266 cells):2.43 μM|Adenosine deaminase (RPMI8226 cells):0.75 μM|Adenosine deaminase (MM1.S cells):0.18 μM
• In Vivo:
  Cladribine inhibits cell growth in primary mast cells (MC) and the MC line HMC-1. It also reduces cell migration ability in CD14+ monocytes as well as CD4+ and CD8+ T lymphocytes. Additionally, Cladribine suppresses cell proliferation in U266, RPMI8226, and MM1.S cells in a dose-dependent manner.
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• In Vitro:
  In adult zebrafish, intraperitoneal injection of Cladribine (0.7-3.5 mM) was found to inhibit the concentration levels of ATP in RBCs (Red Blood Cells).
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• Cell Research:
  The non-radioactive cell proliferation kit is used to determine cell viability. In brief, Human MM cell line U266, RPMI8226 and MM1.S are seeded onto 96-well plates with either 0.1 mL complete medium (5% FBS) as control, or 0.1 mL of the same medium containing a series of doses of cladribine, and incubated for 72 hours. After reading all wells at 490 nm with a micro-plate reader, the percentages of surviving cells from each group relative to controls, defined as 100% survival, are determined by reduction of MTS.(Only for Reference)
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DLBCL, CLL, CldAdo, cell, Cladribine, chronic, Adenosinedeaminase, Adenosine Deaminase, Adenosine deaminase, Adenosine deaminase (MM1.S cells), Adenosine deaminase (RPMI8226 cells), Adenosine deaminase (U266 cells), 2-chlorodeoxyadenosine, 2CdA, 2-Chloro-2′-deoxyadenosine, Apoptosis, mucosa-associated, multiple, inhibit, lymphocytic, lymphoid, lymphoma, hairy, follicular, MCL, mantle, MALT, leukemia, HCL, Inhibitor, tissue, sclerosis

Compound Identifiers

PubChem CID

20279