CK-636

SKU: orb1307381

Description

CK-636 is a cell-permeable inhibitor of the Arp2/3 complex, effectively blocking actin nucleation with species-specific IC50 values. It is widely used in cell biology research to study actin-driven processes like cell motility, endocytosis, and pathogen invasion in both cellular and biochemical assays.

Research Area

Signal Transduction

Key Properties

• CAS Number: 442632-72-6
• MW: 284.38
• Purity: 98.88% (May vary between batches)
• Formula: C₁₆H₁₆N₂OS
• SMILES: Cc1[nH]c2ccccc2c1CCNC(=O)c1cccs1
• Target: Microtubule Associated,Arp2/3 Complex
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (7.03 mM);Ethanol:47 mg/mL (165.27 mM);H2O:< 1 mg/mL (insoluble or slightly soluble);DMSO:40 mg/mL (140.66 mM)

Bioactivity

• Target IC50:
  Arp2/3 complex (human):4 μM|Arp2/3 complex (bovine):32 μM|Arp2/3 complex (fission yeast):24 μM
• In Vivo:
  CK-636 slows down cell migration by inhibiting the formation of lamellipodia at the leading edge of migrating T-cells. In live cells, it suppresses actin polymerization mediated by the Arp2/3 complex. In infected SKOV3 cells, CK-636 reduces the formation of actin filaments. Moreover, a majority of T-cells treated with CK-636 exhibit a turning event upon encountering a zigzag-shaped interface during a letter-shaped test experiment.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CK-636, CK636, CK 636, CK0944636, CK 0944636, CK-0944636, Actin-related protein 2/3 complex, Arp2/3 Complex, Arp2/3 complex (Bovine), Arp2/3 complex (Fission yeast), inhibit, Inhibitor, Microtubule Associated, MicrotubuleAssociated, Human Arp2/3 complex

Compound Identifiers

PubChem CID

588963