Ciprofloxacin hydrochloride

SKU: orb1223617

Description

A broad-spectrum antimicrobial carboxyfluoroquinoline.(In Vitro):Ciprofloxacin (Bay-09867) monohydrochloride (5-50 μg/mL; 0-24 h; tendon cells) inhibits cell proliferation and causes cell cycle arrest at the G2/M phase.Ciprofloxacin (Bay-09867) monohydrochloride shows potent activity against Y. pestis and B. anthracis with MIC90 of 0.03 μg/mL and 0.12 μg/mL, respectively.(In Vivo):Ciprofloxacin (Bay-09867) monohydrochloride (30 mg/kg; i.p.; for 24 hours; BALB/c mice) has protection against Y. pestis in murine model of pneumonic plague.Ciprofloxacin (Bay-09867) monohydrochloride (100 mg/kg; i.g.; daily, for 4 weeks; C57BL/6J mice) accelerates aortic root enlargement and increases the incidence of aortic dissection and rupture by decreases LOX level and increases MMP levels and activity in the aortic wall.Ciprofloxacin (Bay-09867) monohydrochloride (100 mg/kg; i.g.; daily, for 4 weeks; C57BL/6J mice) induces DNA damage and release of DNA to the cytosol, mitochondrial dysfunction, and activation of cytosolic DNA sensor signaling. Ciprofloxacin lactate increases apoptosis and necroptosis in the aortic wall.

Research Area

Pharmacology & Drug Discovery

Key Properties

• CAS Number: 93107-08-5
• MW: 367.8
• Purity: >98% (HPLC)
• Formula: C₁₇H₁₈FN₃O₃·HCl
• SMILES: Cl.OC(=O)C1=CN(C2CC2)C2=CC(N3CCNCC3)=C(F)C=C2C1=O
• Target: DNA gyrase
• Solubility: DMSO: 10 mM

Bioactivity

• In Vivo:
  Ciprofloxacin (Bay-09867) monohydrochloride (30 mg/kg; i.p.; for 24 hours; BALB/c mice) has protection against Y. pestis in murine model of pneumonic plague. Ciprofloxacin (Bay-09867) monohydrochloride (100 mg/kg; i. g. ; daily, for 4 weeks; C57BL/6J mice) accelerates aortic root enlargement and increases the incidence of aortic dissection and rupture by decreases LOX level and increases MMP levels and activity in the aortic wall. Ciprofloxacin (Bay-09867) monohydrochloride (100 mg/kg; i. g. ; daily, for 4 weeks; C57BL/6J mice) induces DNA damage and release of DNA to the cytosol, mitochondrial dysfunction, and activation of cytosolic DNA sensor signaling. Ciprofloxacin lactate increases apoptosis and necroptosis in the aortic wall. Animal model: BALB/c mice. Dosage: 30 mg/kg. Administration: Intraperitoneal injection; for 24 hours. Result: Reduced the lung bacterial load in murine model of pneumonic plague. Animal model: C57BL/6J mice. Dosage: 100 mg/kg. Administration: Oral gavage; daily, for 4 weeks. Result: Had aortic destruction that was accompanied by decreased LOX expression and increased MMP expression and activity. Animal model: C57BL/6J mice. Dosage: 100 mg/kg. Administration: Oral gavage; daily, for 4 weeks. Result: Caused mitochondrial DNA and nuclear DNA damage, leading to mitochondrial dysfunction and ROS production. Increased apoptosis and necroptosis in the aortic wall.
• In Vitro:
  Ciprofloxacin (Bay-09867) monohydrochloride (5-50 μg/mL; 0-24 h; tendon cells) inhibits cell proliferation and causes cell cycle arrest at the G2/M phase. Ciprofloxacin (Bay-09867) monohydrochloride shows potent activity against Y. pestis and B. anthracis with MIC90 of 0.03 μg/mL and 0.12 μg/mL, respectively. Cell Viability Assay Cell line: Tendon cells. Concentration: 5, 10, 20 and 50 μg/mL. Incubation time: 24 hours. Result: Decreased the cellularity of tendon cells. Cell Cycle Analysis Cell line: Tendon cells. Concentration: 50 μg/mL. Incubation time: 24 hours. Result: Arrested cell cycle at the G2/M phase and inhibited cell division in tendon cells. Western blot analysis. Cell line: Tendon cells. Concentration: 50 μg/mL. Incubation time: 0, 6, 12, 17 and 24 hours. Result: Down-regulated the expression of CDK-1 and cyclin B protein and mRNA. Up-regulatedthe expression of PLK-1 protein.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BAY-o 9867 | NSC 620634

Quick Database Links

Gene Symbol

DNA gyrase