Ciliobrevin D

SKU: orb1308452

Description

Ciliobrevin D is a cell-permeable small molecule inhibitor of the AAA+ ATPase motor protein cytoplasmic dynein. It is widely used in research to disrupt dynein-dependent processes, including Hedgehog signaling and primary cilia formation in vivo, as well as microtubule motility and ATPase activity in vitro.

Research Area

Pharmacology & Drug Discovery, Stem Cell & Developmental Biology

Key Properties

• CAS Number: 1370554-01-0
• MW: 392.62
• Purity: 98.09%
• Formula: C₁₇H₈Cl₃N₃O₂
• SMILES: Clc1ccc(C(=O)C(\C#N)=C2/NC(=O)c3ccc(Cl)cc3N2)c(Cl)c1
• Target: ATPase,Hedgehog/Smoothened
• Solubility: DMSO:9.23 mg/mL (23.51 mM)

Bioactivity

• In Vivo:
  In the testis in vivo, Knockdown of Dync1h1 or inactivation of dynein 1 by Ciliobrevin D perturbs spermatogenesis. The use of Ciliobrevin D to inactivate dynein 1 in the testis in vivo perturbs MT organization through changes in the spatial expression of EB1, perturbs F-actin organization, and perturbs distribution of adhesion protein complexes at the BTB, leading to a loss of BTB integrity.
• In Vitro:
  Ciliobrevin D reversibly inhibits melanosome aggregation, however, the non-cilia-disrupting derivative had no discernible effect at comparable doses. Cells treated with Ciliobrevin D exhibits abnormal (unfocused, multipolar, or collapsed) spindles with disrupted γ-tubulin localization in NIH-3T3 cells. Ciliobrevin D addition also reversibly disrupts the pre-formed spindles of metaphase-arrested cells and reduces overall microtubule levels and it similarly abrogates the movement of peroxisomes in Drosophila S2 cells.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Hedgehog, melanosome, Inhibitor, inhibit, Ciliobrevin D, cytoplasmic, Cytoplasmic dynein, dynein, ATPases, ATPase, aggregation, peroxisomes, spindles, Smoothened, γ-tubulin

Compound Identifiers

PubChem CID

136257953