Ciclesonide

SKU: orb1305357

Description

Ciclesonide (Alvesco, RPR251526) is a synthetic glucocorticoid designed for research into obstructive airway diseases like asthma. Its applications include in vitro studies on anti-inflammatory mechanisms and in vivo models investigating pulmonary delivery and efficacy.

Research Area

Metabolism Research

Key Properties

• CAS Number: 126544-47-6
• MW: 540.69
• Purity: 99.94%
• Formula: C₃₂H₄₄O₇
• SMILES: C(COC(C(C)C)=O)(=O)[C@]12[C@]3(C)[C@@](C[C@]1(O[C@H](O2)[C@@H]4CCCCC4)[H])([C@]5([C@]([C@@H](O)C3)([C@]6(C)C(CC5)=CC(=O)C=C6)[H])[H])[H]
• Target: Glucocorticoid Receptor
• Solubility: DMSO:54.1 mg/mL (100.06 mM);Ethanol:54.1 mg/mL (100.06 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (3.7 mM)

Bioactivity

• In Vitro:
  Ciclesonide is effectively taken up into human alveolar type II epithelial (A549) cells and metabolized to its pharmacologically active metabolite des-CIC and fatty acid conjugates of desCIC.
• Cell Research:
  Metabolism of ciclesonide: After initial cultivation, A549 cells are washed five times with 10 ml/dish sterile PBS. An ethanolic stock solution of ciclesonide is diluted 1000-fold with assay medium to generate the initial ciclesonide concentration. The cells are incubated for 1 h in DMEM/0.1% (w/v) BSA (5 ml/dish) containing 0.02 μM ciclesonide as described above in a CO2 incubator. At the end of the incubation period, dishes are washed five times with PBS and incubated for 3, 6, or 24 h at the same conditions in 5 ml/dish fresh medium (DMEM/0.1% (w/v) BSA). At each indicated time point, the medium is removed by aspiration, 5 mM EDTA-4Na/PBS added and dishes are incubated for further 5 min. The cells are detached from the dishes and harvested. Each dish is washed five times with icechilled PBS. (Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Ciclesonide, Alvesco, Glucocorticoid Receptor, GlucocorticoidReceptor, Omnaris, Inhibitor, inhibit, RPR251526, RPR-251526, RPR 251526, Zetonna

Compound Identifiers

PubChem CID

6918155