CHRM2 Antibody

SKU: orb1788044

Description

Purified Mouse Monoclonal Antibody (Mab)

Research Area

Pharmacology & Drug Discovery

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution Range: IF: 1:25, WB: 1:1000, IHC-P: 1:25, IHC-P: 1:25, FC: 1:25
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,κ
• Immunogen: This antibody is generated from a mouse immunized with a recombinant protein.
• Target: CHRM2
• Molecular Weight: 51715 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Muscarinic acetylcholine receptor M2, CHRM2

Immunohistochemical analysis of paraffin-embedded H. heart section using CHRM2. Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. brain section using CHRM2. Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Fluorescent image of SH-SY5Y cells stained with CHRM2 Antibody. Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).

All lanes: Anti-CHRM2 Antibody at 1:1000 dilution. Lane 1: human brain lysates. Lane 2: SH-SY5Y whole cell lysates. Lane 3: U-87 MG whole cell lysates. Lane 4: Y79 whole cell lysates. Lane 5: Neuro-2a whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 52 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing SH-SY5Y cells stained with (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Alexa Fluor 488 goat anti-mouse lgG (166821) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was mouse IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of >10000 events was performed.

Quick Database Links

Gene Symbol

CHRM2

UniProt

P08172