CHRM2 Antibody

SKU: orb1926763

Description

CHRM2 Antibody

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution range: FC - 1:25, IF - 1:25, IHC-P - 1:100-500, WB - 1:500
• Reactivity: Human, Mouse

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,κ
• Molecular Weight: 51715 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Disclaimer: For research use only

Immunohistochemical analysis of paraffin-embedded H. brain section using CHRM2. Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. heart section using CHRM2. Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Fluorescent image of SH-SY5Y cells stained with CHRM2 Antibody. Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).

Western blot analysis of lysates from SH-SY5Y cell line, human brain, mouse brain tissue (from left to right), using CHRM2 Antibody. Diluted at 1:500 at each lane. A goat anti-mouse IgG H&L (HRP) at 1: 3000 dilution was used as the secondary antibody. Lysates at 20 μg per lane.

Overlay histogram showing SH-SY5Y cells stained (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Alexa Fluor 488 goat anti-mouse lgG (166821) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was mouse IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Quick Database Links

UniProt

P08172