Chicken Interferon Alpha (IFNa) ELISA Kit

SKU: orb775955

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Alpha(IFNa).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken IFNa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken IFNa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken IFNa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken IFNa in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Gallus
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 15.63-1000 pg/mL
• Sensitivity: 5.7 pg/mL
• Concentration: 1000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

IFNα1 ELISA Kit, IFL ELISA Kit, LeIF D ELISA Kit, IFN ELISA Kit, IFN-Alpha ELISA Kit, IFNα13 ELISA Kit, IFN-A ELISA Kit, IFNαP22 ELISA Kit, IFN-Alpha 1b ELISA Kit, Interferon Alpha 1b ELISA Kit, Interferon, Leukocytic ELISA Kit, IFN, Leukocyte ELISA Kit, Interferon alpha-D ELISA Kit