Chicken IgG Alkaline Phosphatase Antibody

SKU: orb346063

Description

Chicken IgG Alkaline Phosphatase Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: DOT
• Application Notes:
  Chicken IgG whole molecule alkaline phosphatase has been tested in dot blot and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Key Properties

• Source: Chicken
• Biological Origin: Chicken
• Isotype: IgG
• Purity: This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by conjugation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken IgG, anti-Chicken Serum and anti-Alkaline Phosphatase (calf intestine).
• Conjugation: AP

Storage & Handling

• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.1% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
• Concentration: 1.0 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Chicken IgG alkaline phosphatase conjugated, Chicken IgG alk phos conjugated, Chicken IgY alkaline phosphatase conjugated, Chicken IgY alk phos conjugated

SDS-PAGE of Chicken IgG/IgY Whole Molecule Rhodamine Conjugated (p/n orb346044). Lane M: 5 µl Opal Prestained Marker. Lane 1: Reduced Chicken IgG Whole Molecule Rhodamine Conjugated (p/n orb346044). Lane 2: Reduced Chicken IgG F(c) Fragment (p/n orb346051). Lane 3: Reduced Chicken IgG Fab Fragment (p/n orb2652771). Lane 4: Reduced Chicken IgM Whole Molecule (p/n orb346054). Load: 1 µg per lane. Predicted/Observed size: IgG at 72 and 25 kDa; F(c) at 25 kDa; Fab at 25 kDa; IgM at 75 kDa. Observed F(c) Fragment migrates slightly higher. Other bands: Chicken IgG heavy chain alternative splicing variant at approximately 40 kDa in Lane 1.