Chicken Glucagon (GC) ELISA Kit

SKU: orb779587

Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Glucagon(GC) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glucagon(GC).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 1500 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chicken GC protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken GC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken GC in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Gallus
• Sample Types: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 23.44-1500 pg/mL
• Sensitivity: 7.53 pg/mL
• Concentration: 1500 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

GCG ELISA Kit, GLP1 ELISA Kit, GLP2 ELISA Kit, GRPP ELISA Kit, Glicentin-Related Polypeptide ELISA Kit, Glucagen ELISA Kit, Oxyntomodulin ELISA Kit, Incretin hormone ELISA Kit

Quick Database Links

UniProt

P68259