Chicken Alpha-1-Acid Glycoprotein (a1AGP) ELISA Kit

SKU: orb2667139

Description

Chicken Alpha-1-Acid Glycoprotein (a1AGP) ELISA Kit

Research Area

Gastroenterology & Hepatology, Immunology & Inflammation, Infectious Disease & Virology

Images & Validation

• Application Notes:
  standard: 4000 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken a1AGP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken a1AGP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken a1AGP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken a1AGP in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Gallus
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 62.5-4000 ng/mL
• Sensitivity: 26.7 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

alpha1-AGP; AGP1; a1-AGP; ORM1; OMD 1; Orosomucoid 1

Quick Database Links

UniProt

Q8JIG5