Cell Culture Contamination Detection Kit

SKU: orb1566775

Description

Cell Culture Contamination Detection Kit. The cell culture contamination detection kit is not only used to detect the presence of contaminants but can also identify the type of contaminant, including yeast (and other fungi), Gram-positive bacteria, and Gram-negative bacteria. When using this kit, the sample needs to be stained on two slides: one slide is stained with Calcofluor White M2R, a blue fluorescent dye that specifically binds to fungal cell walls and can be excited by ultraviolet light. The other slide is stained with MycoLight Green JJ98 and Wheat Germ Agglutinin (WGA)-iFluor 594. JJ98 is a green fluorescent nucleic acid dye; WGA is a wheat germ agglutinin that can bind N-acetylglucosamine and N-acetylneuraminic acid residues. Since N-acetylglucosamine is generally more abundant and accessible in Gram-positive bacteria than in Gram-negative bacteria, the fluorescent conjugate of WGA can serve as a probe for Gram-positive bacteria. Therefore, the second slide can differentiate Gram-positive and Gram-negative bacteria. Gram-positive bacteria can be stained with both red and green fluorescence, whereas Gram-negative bacteria only exhibit green fluorescence.

Images & Validation

• Application Notes:
  Microbial contamination in cultured cells can be determined within an hour through fluorescent staining. The approximate type of the stained microorganisms can be directly identified, providing a basis for developing the next step of the solution. The staining solution is in liquid form, requiring no dissolution, and can be directly used to prepare the working solution. The staining is clear, the fluorescence is bright, sensitivity is high, and the results are more accurate.

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Storage & Handling

• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only