PTPRC Antibody

SKU: orb1252617

Description

PTPRC Antibody

Images & Validation

• Tested Applications: FC, IF, IHC
• Reactivity: Human
• Application Notes:
  Flow Cytometry: 0.5-1 ug/million cellsImmunofluorescence: 1-2 ug/mlImmunohistology (FFPE): 0.5-1.0 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)Due to variation in protocol and secondary antibody used, the CD45RA antibody may need to be titered for optimal performance.1. FFPE staining requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: PTPRC/1148
• Immunogen: Recombinant full-length human CD45RA protein was used as immunogen for this CD45RA antibody.
• Target: PTPRC
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Disclaimer: For research use only

Alternative Names

Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, T200, CD45, PTPRC, CD45

IHC test of FFPE human tonsil probed with CD45RA antibody.

Quick Database Links

Gene Symbol

PTPRC

UniProt

P08575