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Description
Research Area
Images & Validation
−| Application Notes |
|---|
Key Properties
−| Source | Mammalian cell |
|---|---|
| Biological Origin | Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) |
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis CD44 at 2 μg/mL can bind Anti-CD44 monoclonal antibody, the EC50 is 64.18-90.91 ng/mL. |
| Tag | C-terminal 10xHis-tagged |
| Molecular Weight | 71.6 kDa |
| Expression Region | 21-650aa |
| Protein Length | Partial |
| Protein Sequence | QIDLNITCRFAGVFHVEKNGRYSISRTEAADLCKAFNSTLPTMAQMEKALSVGFETCRYGFIEGHVVIPRIQPNSICAANHTGVYILTSNTSQYDTYCFNASAPPKEDCTSVTDLPNAFDGPITITIVNPDGTRYIKKGEYRTNPEDIYPSNPTDDDVSSGSSSERSSTSGGYIFHTFSTAHPIPDEDGPWITDSTDRIPATTLMSTSATATETATKRQETQDWFSWLFLPSESKNHLHTTTQMAGTSSNTISAGWEPNEENEDERDRHLSFSGSGIDDDEDFISSTISTTPRVFDHTKQNKDWTQWNPSHSNPEVLLQTTARITADVDRNGTTAHEGNWNPEAHPPLIHHEHHEEEETPHSTSTIQAIPSSTTEETATQKEQWFGNRWHEGYLQTPREDSHSTTGTAAASALTSHPMQGRTTPSPEDSSWTDFFDPISHPMGRGHQAGRRMDMDSSHSTTLQPTADPNTGLVEDLDRTGPLSMTTKQSNSQSFSTSHEGLEEDKDHPTTSTLTSSNRNDVTGGRRDPNHSEGSTTLLEGYTSHYPDTKESRTFIPVTSAKTGSFGVTEVTVGDSNSNVNHSLSGDQDAFYPSGGSHTTHGSESAGHSHGSQEGGANTTSGPVRTPQIPE |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Endotoxins | Less than 1.0 EU/μg as determined by LAL method. |
Storage & Handling
−| Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
|---|---|
| Form/Appearance | Lyophilized powder |
| Buffer/Preservatives | Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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−Recombinant Human CD44 Protein [orb2994984]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 23.1 KDa. Observed: 38-50 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Human CD44 Protein [orb2994009]
Unconjugated
SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE.
Predicted: 49.2 KDa. Observed: 75-95 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgCD44 Mouse Monoclonal Antibody [orb1473982]
FC, IF, IHC, WB
Human
Mouse
Monoclonal
Unconjugated
200 μl, 100 μl, 50 μl, 30 μl

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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis CD44 at 2 μg/ml can bind Anti-CD44 monoclonal antibody, the EC50 is 64.18-90.91 ng/mL.
Quick Database Links
UniProt Details
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Protocol Information
CD44 Protein (orb1477117)
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