CD3E Rabbit Polyclonal Antibody

• Catalog Number: orb10313
• Category: Antibodies
• Description: CD3E Rabbit Polyclonal Antibody
• Target: CD3E
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse
• Predicted Reactivity: Human, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from mouse CD3E (101-189/189aa)
• UniProt ID: P22646
• RRID: AB_10751902
• MW: 23-26 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD3 epsilon; CD3e antigen epsilon polypeptide(TiT3
• Research Area: CD Markers, Cell Biology, Human Lineage-negative,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Sagkan Ozturk, Aliye et al. Short term effects of experimental gastric outlet obstruction and truncal vagotomy on gut hormones Biotech Histochem, (2021)

PubMed: 33722110

Applications

IHC

Reactivity

Rat

Sadighi, Sanambar et al. Down-regulation of immune checkpoints by doxorubicin and carboplatin-containing neoadjuvant regimens in a murine breast cancer model Iran J Basic Med Sci, 24, 537-544 (2021)

PubMed: 34094037

Applications

IHC

Reactivity

Mouse

Sample: Lane 1: Recombinant mouse CD3E protein, C-mFc (HEK293), Primary: Anti-CD3E (orb10313) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 20 kDa, Observed band size: 36 kDa.

Tissue/cell: mouse lymphoma tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-CD3 Polyclonal Antibody, Unconjugated (orb10313) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated (orb868805) used at 1:200 dilution for 40 minutes at 37°C.