CD3E Rabbit Polyclonal Antibody

SKU: orb10313

Description

CD3E Rabbit Polyclonal Antibody

Research Area

CD Markers, Cell Biology, Human Lineage-negative, Immunology, Stem Cell Research

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse
• Predicted Reactivity: Human, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from mouse CD3E (101-189/189aa)
• Target: CD3E
• Molecular Weight: 23-26 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

CD3 epsilon; CD3e antigen epsilon polypeptide(TiT3 complex); CD3E antigen epsilon polypeptide; CD3E antigen, epsilon subunit; CD3e molecule epsilon; CD3e molecule, epsilon(CD3 TCR complex); CD3e molecule, epsilon(CD3-TCR complex); CD3E_HUMAN; IMD18; T cell antigen receptor complex epsilon subunit of T3; T cell surface antigen T3/Leu 4 epsilon chain; T cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T3E; TCRE.

Sample: Lane 1: Recombinant mouse CD3E protein, C-mFc (HEK293), Primary: Anti-CD3E (orb10313) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 20 kDa, Observed band size: 36 kDa.

Tissue/cell: mouse lymphoma tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-CD3 Polyclonal Antibody, Unconjugated (orb10313) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated (orb868805) used at 1:200 dilution for 40 minutes at 37°C.

Quick Database Links

Gene Symbol

CD3E

UniProt

P22646

References

Sagkan Ozturk, Aliye et al. Short term effects of experimental gastric outlet obstruction and truncal vagotomy on gut hormones Biotech Histochem, (2021)

PubMed: 33722110

Applications

IHC

Reactivity

Rat

Sadighi, Sanambar et al. Down-regulation of immune checkpoints by doxorubicin and carboplatin-containing neoadjuvant regimens in a murine breast cancer model Iran J Basic Med Sci, 24, 537-544 (2021)

PubMed: 34094037

Applications

IHC

Reactivity

Mouse